Su Xiankai and Fan Zhen were doing their own experiments, and their eyes swept over Yang Rui's table from time to time.

This is the first time they have met Yang Rui seriously doing an experiment since they entered the "Ion Channel Laboratory".

Although the laboratory director does not necessarily have practical operational capabilities, just as an officer does not necessarily have strong individual combat capabilities, officers with strong individual combat capabilities are obviously more likely to be welcomed by soldiers.

Besides, Yang Rui is really younger, just like a young officer, always susceptible to provocation by soldiers.

Su Xiankai and Fan Zhenlong are newcomers, and Yang Rui is recruited by him. He dare not provoke Yang Rui's authority, but it is still okay to observe secretly.

As they observed, both of them also showed a look of disappointment.

Yang Rui's operational level is too average.

Of course, this is a judgment based on high standards. Yang Rui has actually improved his experimental level a lot compared to his previous experiments. However, since the day he became the head of the laboratory, everyone's expectations for him have also increased.

For example, Xu Zhengping is a middle-aged man who is full and still does not have his own independent laboratory. His experimental level has been trained for more than 20 years. Although many movements are not standard enough because they are not correct when learning, they can continue to do experiments for 20 or 30 years. The non-standard movements have also made Xu Zhengping's experiments smoothly.

On the other hand, Yang Rui could not hide the awkwardness under his hands even though some of his movements were very standard.

"slow."

“Too slow.”

This is the common idea of ​​Su Xiankai and Fan Zhenlong.

As for Xu Zhengping, he had long been used to Yang Rui's experimental movements that were not fast enough. This time, what he didn't believe was Yang Rui's experimental plan.

How many days does DNA sequencing take? What a joke?

Gene sequencing technology began in 1977. At that time, there were methods for chemical degradation to determine DNA sequences, as well as the didn'toxy chain termination method that Yang Rui now did. However, no matter which method it was, it was not easy to use such a technology to do DNA sequencing, with high error rate and low efficiency.

Of course, no matter how low the efficiency is, it is better than the small fragment sequence determination method invented in 1965. The latter is like the potassium ion channel project done by Yang Rui. Not to mention that it depends entirely on luck, the experiment is also extremely difficult.

Xu Zhengping believes that in those top laboratories, some researchers who are super familiar with DNA sequencing may indeed take a few days to make DNA sequencing of plasmids. However, the Ion Channel Laboratory is not a top laboratory, it is not even a provincial laboratory, and Yang Rui is not a researcher who is super familiar with DNA sequencing...

In fact, there are currently no researchers in China who are super familiar with DNA sequencing.

Gene sequencing is indeed a very important biological experimental tool. If the "human gene sequencing" program is the highest peak in biology in the 20th century, gene sequencing can be regarded as the most important experimental tool in biological history.

However, China has always been a hit in the 20th century.

In 1965, sanger invented the small fragment sequence assay and completed the determination of 120 nucleotides of E. coli 5strna. At the same time, the country was busy burning books and beating teachers, so naturally there was no time to learn.

In 1977, practical DNA sequencing methods appeared, and China was busy reprinting books and recruiting teachers, so naturally there was no time to learn.

As an associate professor at Peking University, Xu Zhengping came into contact with DNA sequencing in the past few years. He only did the complete experiment once in total, which was to experience the most important technology of modern molecular biology. Before his project involved gene sequencing, he probably wouldn't do it a second time.

This is the case for domestic researchers. In order to save resources, graduate students learn theoretically when they learn these things.

Xu Zhengping thought about it with his cerebellum that even if Yang Rui had done DNA sequencing, it would only take one and half times.

I can see it by looking at his actions, and it is simply unfamiliar. Using this technique, DNA sequencing takes a few days.

Xu Zhengping deeply believed that Yang Rui had lost money, let alone relying on this to get floating funds, these reagents that cost a lot of money were considered in vain.

Yang Rui himself did not notice the sight of the people around him at all.

This experiment was indeed difficult for him and required full attention.

?Configure buffer and annealing mixture can be done by assistant He Quangui and others, and even centrifugation and ethanol cleaning can be left to assistant. However, when adding the solution, then running electrophoresis, heating, and cooling on ice, it is all Yang Rui's job.

When Yang Rui was studying, the automatic sequencers used for DNA sequencing reached the second generation. Automatic sequencers cannot be said to be fully automatic, but there are many manual steps that can be replaced.

Before the government stopped, ordinary mothers could undergo genetic sequencing and perform various prenatal screening.

But in 1984, there was no current DNA sequencing technology even in the first generation. The United States may not be able to produce the first generation of DNA sequencing machines until next year. If Chinese people can afford it, it will take another two years - just like an Apple phone, you can afford it and you still have to wait for the factory to produce it.

Without an automatic DNA sequencer, manual is complicated.

DNA sequencing ultimately means analyzing the base sequence of DNA fragments. Adenine is A, thymine is T, cytosine is C, and guanine is G. These four letters are circulated in various forms, which constitute the basis of organisms. DNA sequencing is to measure the sorting of these four bases.

Its principle is very simple, but it is not easy to do.

Because bases are invisible, to be sequenced, they must react, and it is the correct reaction.

Accurately allowing the required bases to react step by step, and for theology, it is also the devil's footsteps.

Yang Rui's pace was so slow that Xu Zhengping couldn't stand it. But a few hours later, Yang Rui's electrophoresis was completed and a clear gel appeared in front of several people.

"It's so beautiful..." Fan Zhenlong is older and has more social spirit. He finally found an opportunity to flatter Yang Rui. Although he was lighter, he finally flattered Yang Rui's smile.

Su Xiankai's reaction was a little slower, and he immediately said: "It's pretty..."

No matter how young Yang Rui is, when he is the head of the laboratory, the lifeblood of the people in the laboratory is in Yang Rui's hands. From funding to publishing papers, everyone has to be controlled by Yang Rui. When meeting a better laboratory leader, the funding will be more, the boring tasks will be less, and the paper will be less troublesome. When a paper is published, there will be less trouble. When a laboratory leader with poor character, not only has less funds, but also requires more results. What's more, some laboratory leaders not only have to be the corresponding author himself, but also the first author, but they also dare to sign the author of the paper to people outside the laboratory, making favors and even pure transactions.

Su Xiankai and Fan Zhenlong were just here, and they couldn't figure out Yang Rui's temper, but at least they knew that they were right to follow Mao Lu.

Yang Rui recorded the results and continued to repeat the process just now.

This time, after a few more hours, I still got a beautiful plate of gel and received another round of praise.

Yang Rui repeated again, over and over again.

From morning to evening, Yang Rui did not stop except for eating, and in the afternoon, he even added a set of experiments.

Su Xiankai and others had nothing to say, but Xu Zhengping looked at him straight.

I actually got accurate results every time!

In the era of manual experiments, this is very difficult.

Anyway, this is also a fresh and hot brand new DNA sequence. If it is a repeated experiment, it is of course much simpler to follow other people's answers. Just like the repeated DNA sequencing done by Fan Zhenlong and Su Xiankai, the brand new DNA sequencing will always produce some unwanted answers.

Just the last step of electrophoresis, you often encounter situations where you can’t run out, and then you fail.

Yang Rui was slow in every step, but he did not encounter any mistakes, which made Xu Zhengping hardly dare to think about it.

But in the scene that he dared not imagine, it appeared in front of him now.

This is like a final exam. Only one student scored 100 points. You said he was plagiarized, which would not match. If it was a real exam, then there would be a correct answer. Yang Rui is doing it now, but the S5 fragment sequencing of Tengchong thermophilus is unique in the world. Not to mention that foreign researchers do not have Tengchong thermophilus, they have not even sequenced it. They have published public papers. Xu Zhengping, who often checks the information, is very sure of this.

"It's so beautiful." At 8 o'clock in the evening, Xu Zhengping sincerely praised him as he watched Yang Rui make another clear version of the gel.

Yang Rui smiled and said, "It's just an oil seller."

"You are a pioneering experiment, it's useless to be familiar with it." Xu Zhengping didn't believe what he said.

Yang Rui actually said that he missed the words. He used the answer in his mind to infer the sequence. Of course, it was accurate every time. Naturally, he would not refute it at this time. He pretended to be modest and smiled, saying, "Make another set and I will rest. If you want to go back, go back first."

"Don't worry, let's take a look and learn." Fan Zhenlong flattered again.

"You all have families and families. If you don't go back to accompany your wife, see what's the point of doing the experiment."

"Mental support." Fan Zhenlong's answer was brief and powerful.

"I'll help you. My experiment has been completed, and the reagents prepared today have been used up." Su Xiankai was even more direct.

Many reagents such as buffers need to be prepared immediately. Generally speaking, when you come in the morning, you can decide how much to configure according to the progress. After using it, it is time to end the experiment. Of course, some demanding experiments cannot even be stored for a few hours, so you can only add them in between.

Su Xiankai had already prepared more reagents, but he was not ready to do it until 8 o'clock in the evening. Now he wants to continue his own experiments, but he has nothing to do.

Yang Rui gave him a seat and said, "Then let's spend two more hours with me. I'll finish the sequencing these days. It's good to get tens of thousands of dollars more, right?"

"Of course tens of thousands of yuan will be fine, but it's really hard to finish it in a few days." Su Xiankai told the truth.

Yang Rui smiled and said, "I'm a little tired, but I should be able to finish it."

Su Xiankai couldn't help but say: "The S5 fragment of Tengchong Thermophilus bacteria has a total of 126 base pairs."

126 base pairs are relatively short. Yang Rui made 5 in a day, which is also very fast, but he also knows that it will not be able to complete this project in a few days.

In fact, this is almost here. In foreign countries, many laboratories have sequenced a gene chain and tested it for one or two years. The various strange problems encountered during this period are endless.

It is amazing that Yang Rui did so smoothly today, but if you want to say it smoother and faster, Su Xiankai really can't flatter such an excessive flattery.

Yang Rui had no explanation, but just continued to do it.

No one noticed that his proficiency has increased now.
Chapter completed!