"3-0 line..."

Tanglou directly pressed the crack with his hands and sewed while pressing, and quickly completed partial non-invasive interrupted suturing.

"Gasket...3-0 stitches"

Because the patient's lacerated part of the myocardial wound was not neat enough, Tanglou directly used gaskets and then used mattress sutures.

Tangxian Lake was dazzled by the versatile and appropriate suturing techniques of one hand.

"Replenish blood volume...quickly establish extracorporeal circulation..."

Professor Liu was very experienced and began to direct Deputy Director Qian to perform the operation simultaneously. It must be said that with Tanglou’s fast and perfect suturing, an originally difficult heart rupture repair operation was as simple as suturing an ordinary limb wound.

Generally relaxed.

Ten minutes later, the entire operation was completed perfectly.

"Closely observe blood pressure, heart rate and chest drainage."

"Appropriately replenish blood volume."

"Maintain water and electrolyte balance."

"Pay attention to the use of antibiotics and tetanus antitoxin to prevent infection."

After Professor Liu left the post-operative precautions with Deputy Director Qian, he announced the successful completion of the entire operation.

Tang Xianhu nodded with satisfaction. With his eyesight, he certainly understood how powerful Tanglou's technology was.

After Tang Lou and Professor Liu finished changing clothes and washing up, because Dean Tang had so many things to do, they had a conversation directly in the corridor outside the operating room.

Dean Tang said with great satisfaction: "Professor Liu, Dr. Tang, first of all I would like to thank you both on behalf of my nephew. The operation was very successful."

Professor Liu and Tang Lou naturally said that they were both doing their jobs and they were very honored to be able to operate on the dean's nephew.

"Professor Liu, after I have considered Dr. Tang's topic, there is no problem at all. You can arrange the project report according to the normal time node, and I will attend later. At the same time, Dr. Tang entered the laboratory in advance to conduct ICR

I approved the induction of mice."

"Really, thank you so much, Dean."

Tanglou did not expect that Dean Tang would agree so readily.

"Do a good job."

Dean Tang encouraged Tanglou again and rushed to attend a meeting.

For this reason, Professor Liu also specifically gave instructions on what to pay attention to in the laboratory. As for the rest of the laboratory regulations, Liu Qiqi will of course help.

...

...

With the authorization of Dean Tang and the arrangement of Professor Liu, Tanglou entered the laboratory the next day.

The laboratories of Zijingang University are leading in the country and equipped with various equipment.

At Tanglou's request, Wu Qingguo also cooperated very well and sent the first batch of conventional Kunming white mice that Tanglou needed to the laboratory.

Although Tanglou has verified the best induction plan in the system laboratory, as a real-life experiment, in order to be convincing and credible, Tanglou still designed the control group and experimental group according to the normal process.

, and established a statistical analysis model.

As for equipment, Tanglou also prepared in advance the NTS-1300 constant temperature oscillating water tank from Tokyo Rika Instruments Co., Ltd. and the CX-41 optical microscope from Olympus through the help of Liu Qiqi.

As for the reagents, Tanglou also configured them strictly according to the test procedures and made detailed records. Obviously this is a very huge workload.

Of course, these reagents and materials were paid for by Tanglou himself.

Professor Liu only allowed him to use various laboratory equipment.

After all, Tanglou's project has not even started yet. According to Tanglou's own thinking, there is no project funding. Professor Liu also persuaded Tanglou to apply for funds, but Tanglou rejected it.

After all, for the sake of his own big plan, Tanglou was still willing to give up the money, even though it was very painful!

Tang Lou really wants to become a medical cosmetologist. After all, major U resection surgery, double eyelid surgery, and rhinoplasty surgery are all low-risk and high-yield, especially with his textbook-level basic skills. As long as Tang Lou is willing,

Those big celebrities didn't come here one by one.

Jiang Shu had intended to undergo a tightening surgery before, and Yang Jinli had also asked whether Tanglou could undergo a bear augmentation surgery, not to mention that Zheng Shuang, who was undergoing plastic surgery every day, could use the latest version of the nose, even the sharp ones.

Scratched the glass.

Of course, Tang Lou wanted to give her the whole brain.

Tanglou began to prepare the solvent 0.9% sodium chloride injection very carefully. The positive reagent CP (purity ≥ purity 1) was purchased from Sigma Aldrich.

The preparation concentration is 4 mg/mL; fetal calf serum (FBS) was purchased from GIBCO.

Potassium chloride (KCl) was purchased from Sigma Aldrich

Use deionized water to prepare 0.075 mol/L. Giemsa dye was purchased from Sigma Aldrich.

And the most important thing is that before staining, 1 part of Giemsa stock solution is mixed with 9 parts of 1/15 mol/L phosphate buffer (1/15 mol/L Na2HPO4 solution and 1/15 mol/L KH2PO4 solution 1:1, pH 6.8

) to prepare a 10% Giemsa application solution.

...

Looking at these reagents purchased from companies around the world, Tanglou felt heartbroken again. Fortunately, he had mastered the final optimal ratio. Otherwise, if he had followed the normal experimental process and just found the optimal ratio, he would have to

It wastes a lot of reagents and time, but it may not be successful.

After preparing the reagents, Tang Lou began to take out the mice that Wu Qingguo had sorted in advance.

Each batch of Tanglou mice is limited to 36 mice. These mice are all 7-8 weeks old, weigh 30-32g, and are all male.

Although these mice are not in the best condition in Tanglou's opinion, they are from the animal experimental base of Jin Group after all. These mice have passed the ethical review of the Experimental Animal Welfare and Ethics Committee of the National Center for Drug Safety Evaluation and Testing.

At the same time, he also has an animal production license, so this experiment in Tanglou is very rigorous and formal in all aspects, and can be included in a subsequent formal paper.

Although Tanglou did not graduate from a top medical school, he was still a 211 graduate and was still very familiar with the basic skills of experiments.

Tanglou used TOXSTAT2006 data statistical analysis software to randomly group the subjects to control individual differences in grouping.

After that, clinical symptom observation and body weight measurement will be carried out strictly in accordance with the formal procedures.

Because it is in a real laboratory, there is no double-speed function. Tanglou can only administer medication honestly, once every morning and afternoon, and observe in cages, including whether there is death, dying, activity status, appearance and being

Hair, presence of trauma, feces condition, etc.; animals were weighed before administration, during administration and before dissection.

In the next week, Tanglou entered into a very fulfilling experiment.

Although the results were already known, for the sake of a rigorous database in the future, Tanglou still analyzed and recorded the relationship between the types of chromosomal aberrations at different dosage concentrations and sampling time points.

During this period, Wu Qingguo also came to the laboratory every other day, firstly to deliver mice to Tanglou, and also to observe the experimental process of Tanglou curiously. By the way, he also reported the current situation of Tanglou, as well as the records and preparation of medicines in Tanglou.

All kinds of beautiful photos of breathing and dazed were sent to Kim Yuna.

Finally, on the seventh day, Tanglou completed the first batch of chromosomal mutagenesis of the stable male parent of ICR mice.

Under the arrangement of Tanglou, Wu Qingguo sent the first batch of male parents to the experimental base and began to breed the first inbred strain.

...

...
Chapter completed!