Just when Chen Changan was busy with the R&D work of his home personal assistant, Wang Jia's group in the research center officially established a dental regeneration project after receiving the funds approved by Chen Changan and began related research and development work.

Wang Jia's laboratory was once again filled with mice of different ages, from childhood to old age!

To study stem cells of teeth, you have to start from mice.

In recent years, increasingly stringent ethical review and tension in the relationship between doctors and patients have made it increasingly difficult to pass human experiments and applications for extracting some research objects from the human body.

If you want to extract tooth pulp stem cells or other stem cells directly from human teeth, it is difficult to pass the ethical review, so Wang Jia had to start with the mouse.

Several young mice were given anesthetics by researchers and several baby teeth were removed.

The tissue was extracted from several deciduous teeth, and after culture and amplification, the researchers successfully extracted the dental pulp stem cells from it.

Dental stem cells are a good thing. After in vitro culture for a period of time, researchers clearly found that this cell showed enhanced osteogenic/dentination differentiation ability.

The researchers also found that this stem cell composite transplantation with TDM material can form dentin and neurovascular-like structures in the body, imitating natural teeth, and can regenerate dentin, capillary network, peripheral neural network and other dental cells.

This kind of cell has been discovered in medicine and is named by the medical community: pluripotent pulp regeneration stem cells.

As long as stem cells with the word "poly energy" are all very awesome stem cells, one cell can differentiate into various organs and tissues!

So does the regeneration of stem cells with multipotent pulp!

After careful research, it can be confirmed that this type of cell only exists in the baby teeth and wisdom teeth.

As we all know, people have two sets of teeth. The teeth in childhood are baby teeth. By adolescence, the baby teeth have basically fallen off and have been replaced with a second tooth, permanent teeth.

If the permanent teeth break again, after removal, the third pair of teeth will not grow, and it will disappear.

Moreover, permanent teeth are very mature teeth, and there is no longer the presence of multipotent pulp regenerating stem cells inside.

However, there are four other teeth in adults' teeth that contain pluripotent pulp regenerating stem cells, that is, wisdom teeth.

Wisdom teeth are relatively late-developed teeth and are also four degenerated teeth.

Because wisdom teeth develop relatively late, although they only grow by adults, strictly speaking, wisdom teeth are also a type of baby teeth, and are the first teeth to grow!

As long as it is a baby teeth, the dental tissue will contain multipotent pulp regenerating stem cells!

According to Wang Jia's cultivation test, only one cell is needed to extract the deciduous tooth stem cells, which is enough for a person to use for the rest of his life.

Through refined operations, the problem of stem cell contamination in the deciduous teeth without the addition of antibiotics can reach more than 10 million cells in the first generation and tens of millions in the second generation can be expanded.

Tens of millions of pulp regeneration stem cells are enough to repair and regenerate a lot of teeth, which is definitely enough for normal people to use for a lifetime, and may not be able to use them all.

Even if some people still don’t have enough, they can still proliferate the third generation of pulp and regenerate stem cells.

As long as there is a tooth that can extract tooth pulp and regenerate stem cells, there is a possibility of teeth regeneration.

However, this study found that it was not perfect.

Although there are currently many stem cell banks and dental stem cell collection banks approved by national competent authorities across the country.

However, few people really have the awareness of preserving stem cells. Most adults have not preserved their own dental tooth pulp regeneration stem cells in the stem cell library.

In this case, even if the technology of using pulp to regenerate stem cells to repair and regenerate teeth is eventually conquered by Wang Jia and commercialized, most people will not experience the benefits of this technology.

Because this type of tooth regeneration must be done by using your own stem cells. Using other people's pulp to regenerate stem cells will undergo rejection reactions, which the body does not accept.

This is very embarrassing. Although theoretically, using pulp regeneration stem cells can regenerate teeth for everyone, most adults have replaced their deciduous teeth and their wisdom teeth have been removed. Even if they have not been removed, some people may not have wisdom teeth innately, and they do not have pulp regeneration stem cells available.

This technology may be useful to the next generation. As long as we build a complete dental stem cell library from now on to preserve the tissue of the baby teeth for every child during the dental change period, they will no longer be troubled by dental diseases when they grow up.

But what to do with billions of adults around the world...

Obviously, the dental conditions of adults are more difficult and more serious than those of children.

And it is also a larger market...

After all, children are not growing up yet, and their baby teeth have not been replaced. It is still early to have problems with their teeth. Nowadays, most of the adults who are suffering from dental diseases are 25-50 years old...

It would be a waste to give up such a large market and re-cultivate the next generation of markets. Not to mention that Chen Changan could not accept it, even Wang Jia himself could not accept this result.

After she came up with the research results, she gathered all the researchers in the project team and held another discussion meeting.

The question discussed is very simple, how can we solve the problem of missing stem cells in adults?

Nearly a hundred researchers sat in the conference room bustling and discussed for a long time. In the end, everyone found that there was only one way.

A representative raised his hand and summarized his speech: "Professor Wang, can we try to induce pluripotent stem cells and degenerate the somatic cells back to the stem cell state?"

After thinking about it, the only possible way is to transform the dental cells that have been developed in adults back to their original stem cell state. Only this method can allow adults to obtain dental stem cells.

Wang Jia frowned and felt it was difficult.

Induction of pluripotent stem cells is not a new theory. The global medical community has invested at least 40 years and countless funds in this field for research.

However, the effect is not very gratifying. After more than 40 years of continuous research and experimentation, only a few research institutions in Japan and Europe have successfully induced differentiation of human somatic cells back to stem cells.

Although they succeeded, they used some very complex means, and there were very few successful cells, which were as few as the single digits, and could only induce a few pluripotent stem cells.

This result is better than nothing. Although it is very exciting, it is basically useless for practical applications.

This is the result that took the time and energy of several generations of top experts in the field of life sciences.

Wang Jia doesn't think that with her dozens of people, she can do more in a short time than these experts who have studied stem cell for decades, and can develop mature induced pluripotent stem cell technology...
Chapter completed!