Four hundred and sixty-five chapters tooth embryo
At around nine o'clock in the morning, Wang Jia arrived at the Gusu Children's Hospital on time with seven or eight subordinates, carrying several medical boxes.
Before this, Wang Jia had already placed some equipment for dental regeneration projects in advance in a disposal room specially vacated by the dentistry.
In fact, there are not many equipment, mainly two devices with a height of humans, one of which is a cell cultivation device for culturing dental stem cells, and the other is a 3D cultivation device.
The usage is not complicated.
After meeting the three young volunteers, Wang Jia simply chatted with the three parents and took them to this disposal room together.
"Student Gu Zhengan has well-preserved dental stem cells, so we will conduct clinical trials for Gu Zhengan first." Wang Jia reached out and took a culture dish containing dental stem cells from Gu Zhengan's mother's hand. This was taken out of the stem cell bank early in the morning and was frozen throughout the process.
Now after treatment and returning to normal temperature, the cell activity has returned to normal.
Wang Jia carefully placed the pulp stem cells that belonged to Gu Zhengan stored here into the first culture tank in the cell cultivation equipment.
At present, cell culture and proliferation technology is very mature.
A single pulp stem cell can be cultured in P1 generation, and can be expanded to tens of millions of pulp stem cells. After proliferation, it is enough for an adult to replace all the mouthful teeth.
However, this culture takes time and cannot proliferate so many pulp stem cells overnight.
After Wang Jia put Gu Zhengan's dental stem cells into the culture equipment, he signaled the two dentists from the children's hospital to extract the pulp stem cells for the remaining two volunteers.
Although the parents of these two children did not have an advanced awareness and saved the pulp stem cells in their baby teeth in advance, the good news is that their baby teeth have not fallen off.
Generally, when children are 6-8 years old, their incisors and canine teeth will fall off first. This is why children of this age often see air leakage in front teeth, which means they are in the tooth change period.
At the age of 10-12, you will start to replace larger teeth. Usually around the age of twelve or thirteen, all teeth molars will fall off and replace them with permanent teeth.
At this point, all the deciduous teeth in the oral cavity have been replaced for permanent teeth, and there are no dental pulp stem cells in the permanent teeth.
However, people who have completely replaced their teeth are not without the chance to obtain dental stem cells again.
In addition to the baby teeth, there is also a type of tooth that has tooth stem cells, that is, the third molar, commonly known as wisdom teeth!
The third molar is a degenerated tooth. Due to the degeneration, the gum is not big enough to accommodate the third molar, which causes the tooth to sprout relatively late. Moreover, there is often no place to grow because of the insufficient gum, so the wisdom teeth are painful and need to be removed.
Because wisdom teeth are also newborn teeth that grow for the first time, although they are permanent teeth, they also have pulp stem cells. Therefore, if adults want to regenerate their teeth, they should pray that they will grow wisdom teeth, and come to the hospital to remove them immediately when wisdom teeth grow and extract the pulp stem cells in it.
For the time being, there are only two ways to obtain dental stem cells.
Under the comfort of the two dentists, the two children lay on the tooth extraction bed. After two clicks, one of them was pulled out and the teeth were grinded.
Their second molars were still deciduous teeth. After being extracted, several of Wang Jia's subordinates immediately took them and quickly separated the two deciduous teeth with blood. After extracting their respective dental stem cells, they immediately placed them in the second and third culture tank of the culture equipment.
"Okay, after the extraction of the pulp stem cells is completed, it will take about one month to proliferate to meet the number of teeth regeneration. After one month, we will conduct 3D culture. At that time, the three volunteers come here again. We will perform subcutaneous implants for you, and then a new tooth will grow!"
Wang Jia nodded with satisfaction, feeling very good, his eyebrows and eyes were full of smiles.
It only takes another month to conduct a real dental regeneration project test. If this test is successful, the entire dental regeneration project can basically be declared successful, and the subsequent work can be completed by the subordinates.
And she can participate in the regeneration of the real big project organ in Yun Heng!
When Wang Jia thought of this, he couldn't wait~
............
One month later, after the culture equipment successfully proliferated the pulp stem cells of the three volunteers to a level that was sufficient for teeth regeneration, Wang Jia once again notified the three volunteers to come to the hospital to prepare for the next stage of treatment.
This time, the three children are fine, and the three groups of adults are even more excited than last time. Last time, they just came to do some preparation work, and this time they will have to officially regenerate their teeth. Success or failure is here!
The parents of the three volunteers were both anxious and nervous, and kept asking the doctors and nurses in the dentistry department to say all kinds of good words and guarantees they didn't have money to say.
The doctors and nurses who were held back could only deal with it with a wry smile. They could not guarantee anything about this. It was the people of Ruikang Group who did the teeth regeneration, not them. They didn’t even know the technical points at all, so where could they draw any conclusions on it.
They could only persuade them to relieve the nervousness of parents, say some good things that don’t cost money, and praise Ruikang’s skills...
And just when the parents were nervous, the three young volunteers in the clinic were also a little nervous.
No matter what, they are just a group of children in their early teens, and they will be very nervous when they are surrounded by uncles and aunts wearing white coats and masks.
Seven or eight staff members were surrounding them for various detailed oral examinations. In a large cultivation equipment next to them, three small dots that had formed the prototype of the teeth were lying quietly in the culture medium.
These three small dots are simulated by the three dental embryos cultivated through 3D culture technology.
Yes, tooth has embryos.
Although they are small, they should have enamel, dentin, cementum, and pulp. They only need to be implanted under the skin of the patient's pulp cavity and rooted in the gums to grow into a healthy tooth by themselves!
Three little volunteers lay nervously on the dental bed, staring at their little eyes with some frightened eyes, and watched several dentists take out the teeth embryos from the Petri dish and put them in their mouths.
Chapter completed!