chapter 93
In the laboratory, more than 20 people stood around the operating table, but they were extremely quiet.
Everyone was watching Wang Jia pump bone marrow at one tiny mouse after another.
More than a dozen teenage mice that had just finished marrow and had not yet woken up from the anesthesia were lying in the "Awakening Box" placed not far from the operating table.
Everyone watched helplessly as Wang Jia went from being very cold and almost expressionless to being more excited as he twitched, the smile on the corners of his mouth became stronger and stronger, and his expression became more and more enjoyable...
All the onlookers present felt a chill spread from the bottom of their hearts, and there was an indescribable strangeness.
Chen Changan gently stabbed Guan Kou in the arm and whispered in a low voice: "If you change the puncture needle on Wang Jia's hand to a scalpel, it will not look like the kind of evil scientist who loves to do human experiments as described in horror."
Guan Kou held the glasses frame and said with a trembling voice: "Mr. Chen, are all biology people so "invested"?"
She really couldn't think of any words to describe Wang Jia's current state, so she could only use the word "investment" to explain...
"No, my former female classmate didn't dare to dissect the little white rabbit during the experiment class, let alone look at the even more disgusting mice and frogs. They were all rejected. This person was not an ordinary person at first glance..."
Although Chen Changan and Guan Kou had tried their best to whisper in a low voice, the laboratory was so quiet that it could be heard even a needle fell to the ground.
So the conversation between the two was still unblocked to Wang Jia's ears.
She slightly restrained the smile on her face and instantly became very cold again.
"I don't even dare to kill a test rabbit, so I'd better change careers as soon as possible, it's not suitable for biomedical science."
Wang Jia said something, but he had no intention of stopping in his hand. He was so scared that he inserted the puncture needle into the mouse's lower spine and skillfully pumped a few milliliters of bone marrow.
Her actions were even more scary with verbal words...
Chen Changan shivered and immediately explained: "Professor Wang, I have nothing else to mean, I just sigh at your skillful technique. Don't get me wrong!"
"Oh, it's okay. I like to do experiments more and enjoy the state of doing biological experiments. Sometimes it's really scary. You are not the first to think so, I'm used to it."
Wang Jia continued to change into another little white mouse without looking back, not caring about the strange look that everyone casted at her.
She had become accustomed to this kind of experience when she was in college.
I still remember the first time I took animal physiology and anatomy class at school.
Other students were resisting dissection of the little white rabbit, and were wailing and doing psychological construction. Without hesitation, she raised her hand and cut open the abdomen of the test rabbit, and took out all kinds of heart, liver, spleen, lungs and kidneys. She still remembered the frightened expressions of the students at that time, the high decibel screams, and the professor's indescribable expressions.
After that, she left a very "cool" nickname in the Department of Biomedicine at Sun Yat-sen University.
"Cold Blooded Ripper!"
Later, due to her excellent grades and experimental ability, she completed all university courses ahead of schedule in less than two years, applied for a direct doctorate of the school, and clapped her hands and left the university department of Sun Yat-sen University.
However, to this day, her name as a cold-blooded Ripper is still spread at Sun Yat-sen University. Every freshman in the Department of Biomedical Sciences will know her name from the lecturer...
Not only that, her fellow disciples who were studying for a doctorate also made her very excited and enjoyable behaviors, and she sighed more than once, and spread it everywhere as a gossip.
After being discussed a lot, Wang Jia became accustomed to it over time.
Just as everyone was watching with strange eyes, Wang Jia put down the puncture needle with satisfaction after taking the bone marrow fluid from sixteen little white mice, clapped his hands and said, "Okay, so much should be enough."
Chen Changan walked up and took a few glances. Wang Jia had almost 20 to 30 milliliters of bone marrow fluid. It was estimated that a large number of hematopoietic stem cells could be extracted from it, which was enough for the current experiment.
"Okay, these are enough. There are just sixteen tubes. You will divide them into sixteen groups. Extract all the hematopoietic stem cells in the bone marrow fluid as soon as possible, and then put them in the culture medium to cultivate, so don't kill them."
If the cells are ex vivo, under normal circumstances, even cancer cells can only survive for hours or even dozens of hours. The proteins in the cells will deteriorate and the cells will die.
However, if it is in a laboratory environment, cells with the best living conditions. Cells with self-dividing functions, such as stem cells, can generally survive for about three to five months. The specific depends on the replication and differentiation ability of the cells.
Cells like cancer cells that have infinite replication characteristics can achieve immortality in a laboratory environment!
For example, in 1951, tissue samples taken from an American woman's tumor were copied and transmitted to laboratories around the world. Until now, her cancer cells have been active in biological laboratories around the world and are important samples for studying cervical cancer.
What Chen Changan wants to do now is to inject c-myc genes that can achieve infinite replication characteristics through artificial means, so that hematopoietic stem cells can also be replicated infinitely.
This is the first step that must be taken to achieve artificial hematopoiesis!
Otherwise, normal hematopoietic stem cells cannot be divided infinitely, and even if Chen Changan creates artificial hematopoietic equipment, he will not be able to obtain an unlimited supply of hematopoietic stem cells.
The hospital cannot find volunteers to donate bone marrow at any time, right? Bone marrow extraction is not a comfortable thing. Many patients who have undergone bone marrow transplant surgery are in pain after the operation.
Of course, there is another way, which is to extract hematopoietic stem cells from the baby's placenta, but this is very moral and requires ideological work for pregnant women every time. The amount of blood that can be extracted is also a drop in the bucket compared to the amount of blood used in the entire hospital.
The only way to achieve large-scale mass production of hematopoiesis is to give hematopoietic stem cells the characteristics of infinite replication!
As long as hematopoietic stem cells can replicate infinitely, you only need to prepare sufficient culture medium, and based on one gram of hematopoietic stem cells, you can replicate and divide unlimitedly, which is really endless.
In Chen Changan's mind, a mature artificial hematopoietic device only needs to provide one gram of hematopoietic stem cells with the c-myc gene, and then inscribe the transcription factor into the hematopoietic stem cells.
Induced by special rays, hematopoietic stem cells will divide and proliferate infinitely. After maturity, they will then transform into red blood cells and platelets, and eventually become mature red blood cells and platelets, stored in a blood reservoir of the device.
After the blood cells in the blood reservoir are full, the medical staff only need to replace the blood reservoir, and hold the blood reservoir full of blood cells, separate and store the blood cells inside into the blood bank, and wait for use!
Very convenient and popular!!
As long as Chen Changan can manufacture this near-future artificial blood equipment, the blood shortage problem that has plagued mankind for hundreds of years will no longer exist!
Chapter completed!