Tang Shun plans to open 10 incubators at the same time. The experimental scale of 10 incubators requires about 100 million yuan in scientific research funds, which is already an astronomical figure for medical projects.
However, Yang Ping felt that Tang Shun was too conservative. 10 incubators would definitely not be enough, and additional incubators would definitely be needed in the future. Rather than adding in mid-stream, it would be better to do it all at once. Yang Ping suggested that Tang Shun open 100 incubators at the same time.
According to the current success probability, only a few dozen of 100 incubators can be successful. Of these dozens, one specimen needs to be taken for section observation at each stage, so the number of specimens consumed is also very large.
But for 100 incubators, scientific research funds require 1 billion. Professor Yang is confident.
Since Professor Yang was so supportive, Tang Shun was no longer stingy. He immediately expanded the scale of the experiment and opened 100 culture vessels at once. The stem cell laboratory space that currently occupies one floor is definitely not enough. In order to place so many culture vessels
, the stem cell laboratory adds another floor.
Let alone adding one floor, even if one building is added, in Sanbo Hospital, it is only a matter of Yang Ping's words.
As for the funding issue, it is not a problem at all. Yang Ping’s special scientific research fund, the European Duke Fund’s donation alone is one billion euros. Although the money is paid in installments, there are hundreds of millions of euros already received, not to mention that there is another person standing behind him.
Consortium---Anning Group.
Even without these donations, with the current financial resources of Ruixing Group, it is not difficult to spend more than one billion.
According to Yang Ping's request, Tang Shun launched the entire experiment.
The way cells divide is exponential division, which is very fast. Soon, the stem cells in the culture vessel appear visible to the naked eye, which are very small, only as big as a sesame seed.
Out of 100 incubators, 39 successfully cultivated shaped organ embryos, and 61 only cultivated scattered cell piles.
Tang Shun took 31 successful specimens, one specimen from each period, for observation, and finally made slices and put them under a microscope for structural analysis.
Moreover, the digital human technology of the Digital Medicine Laboratory of Nandu Medical University was used to compare and study these specimens, and their structures were clearly understood.
It can be confirmed that this is not a cluster of scattered cells, but an organ with its own structure and shape. Sesame slowly turns into mung beans, and mung beans turn into peas. When the cultured muscles are the size of peas, the shape of the muscles can already be seen with the naked eye.
.
"Dr. Tang, look, they are plump in shape and have a smooth surface. They are completely different from other cell piles." The researcher also saw the difference.
Tang Shun stared excitedly at the muscle "seedlings" in the transparent cabin: "Even with the naked eye, it can be judged that this is not a scattered pile of cells, but a muscle, but it is still very small and has just begun to sprout."
No matter how small, there is still a big difference in appearance between a pile of bricks and a small house made of bricks, and even a young researcher can recognize it with the naked eye.
When they heard Tang Shun say that this was a muscle, several researchers opened their eyes wide. They couldn't believe that their laboratory could really grow a muscle and artificially grow a separate organ in vitro. This is currently the world's
This is something that many top laboratories in the world cannot even imagine. At best, they are cultivating organs in animals for human transplantation through genetic modification and parasitism.
For example, genetically modified hearts are grown in pigs and then transplanted into humans. Several such surgeries have been performed in the United States.
Unfortunately, this method is not yet mature, and the longest survival time for patients after heart transplantation is only a few months.
Tang Shun carefully took out a formed muscle "germ" with tweezers, cut it into slices and put it under a microscope for careful observation.
The structure has become clearer and clearer. Sure enough, this is not a bunch of cells, but a muscle built by cells. Although it is small, its structure is exactly the same as that of a normal muscle, and there are actually blood vessels and nerves that shuttle through it.
Through observation, the muscles cultured separately in this way have similar structures to those in embryos.
Because this muscle relies on nutrient solution to provide oxygen and nutrients, its vascular system has not yet been perfused with blood and is in a non-filled state. Therefore, the blood vessels are not in an ideal tubular shape, but a flat potential cavity structure, which confirms
A rule of human body science - structure and function are adapted, and function will in turn affect structure.
After all, this is an in vitro culture. All oxygen and nutrients come from the nutrient solution. In the future, when the muscles grow to a certain shape, a special artificial heart-lung machine will be used to help the muscles establish an artificial circulation. This artificial circulation machine will reserve various resources.
The arterial and venous interface of the caliber is connected to the dynamic and static veins of the muscles through artificial pipelines to form an artificial circulation.
Under the influence of artificial circulation, flat blood vessels will gain filling tension. After a period of time, the blood vessels will slowly return to their pipe structure, and the thickness and elasticity of the blood vessel walls will also tend to normal.
Yang Ping's laboratory plan also mentioned this and gave specific guidelines.
Professor Yang's experimental plan actually predicted all the problems related to the experiment and provided solutions. This requires strong and rigorous logical thinking skills.
In theory, it is beyond the reach of the human brain, but he did it.
Tang Shun was amazed.
According to this calculation, the experimental equipment for muscle cultivation in the future should not be these equipment, but a set of equipment should be specially designed to meet the needs of the experiment.
While Tang Shun was thinking about this problem, Yang Ping called him and asked him to go to the office and deliver another A4 paper to Tang Shun.
"This is the drawing of the experimental machine. Because this is our first experiment, we can only use off-the-shelf machines. We will need these specialized machines for the next experiment. This is a complete set of equipment drawings. Take a look.
, and then contact Ruixing Company and leave it to their engineers to develop and design it.”
This chapter is not finished yet, please click on the next page to continue reading the exciting content! Tang Shun held this drawing in his hand and felt it was heavy. What he was holding was not a stack of paper, but priceless black technology.
If he continues to follow Professor Yang, he will become the world's top stem cell scientist in a few years, surpassing his mentor.
"Professor, is there anything else?" Tang Shun put the A4 into his briefcase with trembling hands.
Yang Ping thought for a while and said: "I want to start a medical journal. Let's think about the name together. Our goal is a world-class journal, so the name must also be world-class. I plan to publish the final results of this experiment in
in your own journal.”
Want to start your own journal? Still a world-class one.
Any shocking move is as simple as playing a game with Professor Yang.
Do you know how difficult it is to start a world-class medical journal? Moreover, it is very difficult to establish a journal in Chinese and gain recognition from scholars around the world.
So far, it seems that there is no truly world-class journal in our country.
"Go ahead, you have worked hard during this period. The scale of the experiment is relatively large. You must take the time to think about the name of the journal. Remember, this is a world-class journal."
The professor is always so confident in his words, there is no doubt that he will be able to do what he wants to do.
---
When Robert returned to the United States, it took several days to adjust.
He was very satisfied when he saw the banners hanging on the walls of his clinic. This was the effect he wanted.
It's just that the words on all the banners are the same, and even the signature of the giver is exactly the same.
Robert looked at dozens of identical pennants and frowned: "Barrett, are all pennants like this?"
Barrett excitedly expressed his gratitude: "Yes, sir, I asked them to customize the banner according to the template you gave me. If I guess correctly, all the shops in Chinatown now have this kind of banner. Many clinics nearby have also taken the initiative to learn from us.
, after all, our clinic is the first-class in the United States, so the layout and decoration style of the clinic have become a reference for the industry. Now, they have followed us and started hanging pennants, and they are all the same. They are all this kind of pennant. Only this kind of pennant can be used.
It’s genuine.”
Robert touched his red nose. It was really sloppy. At that time, he sent the template to Barrett and ordered him to follow it. All the words on it were not hidden or modified, and no follow-up instructions were given to Barrett.
According to the instructions, Barrett cannot be blamed. He was too busy with work in China at the time and made mistakes in his instructions.
It is definitely too late to change it now, not to mention that this kind of pennant has become a popular element.
It's a done deal, just make mistakes.
"Barrett, you are good." Robert praised his smart assistant.
Barrett was praised by Robert and felt that all the efforts during this period were worth it. You must know that he went to Chinatown many times to find this kind of shop that makes pennants.
Finally, a small advertising company was found who was willing to take the order. The boss asked them if they were sure that all the banners were the same, and pointed out the small print next to them. Barrett pretended to be an expert - of course he was sure, and he would not change a word.
The other party offered a banner of US$500 per banner, claiming that these materials must be imported from China, so the price could not be too low. Barrett abruptly lowered the price to US$400 per banner, saving the clinic a lot of money.
Now that the boss is finally back, several agents of sports stars have contacted the clinic, hoping to schedule surgeries, including NBA stars, boxers, Canadian skiers, and too many to count.
The boss has been staying in China, and Barrett was worried to death. Now that the boss is back, all problems have been solved.
Barrett served Robert coffee. Robert waved his hand, took out a can of West Lake Longjing from the drawer, and said mysteriously: "We will drink this from now on. Come, I will teach you how to make tea."
Robert taught Barrett how to make tea step by step, and then taught him about the culture of tea.
"Also, sir, our entire clinic has a very high enthusiasm for learning Chinese and has done a good job in popularizing Chinese. Our work uniforms, hand washing uniforms, nurse uniforms, wherever text is needed, we use English and Chinese bilingual, especially
Our work clothes have all been embroidered with Chinese characters - Brother Luo Clinic. According to your instructions, I have applied for trademark registration of Brother Luo Clinic and it has been approved." Barrett reported on the recent work progress.
Robert gave a thumbs up: "Very nice!"
Barrett became more and more enthusiastic:
“Do we need to change our sign?”
Robert said without thinking: "Of course, write Brother Luo Clinic in Chinese on the top and the original name in English on the bottom."
"Okay, I went to Chinatown to find someone to write a sign. For our clinic, I risked my life. Now I am a frequent visitor to Chinatown. There is an acupuncture doctor there. It is said that he can write with a brush and he writes very well. I kept his
Telephone."
"No, I'll write it myself."
"Boss, can you write with a brush?"
"I'll give it a try. I've seen other people write about it in China. It shouldn't be difficult. I originally wanted to learn it, but I didn't have enough time."
"Boss, will our patients recognize the Chinese characters on the sign? Will it affect patients' perception of our clinic?"
"If you don't know these Chinese characters, you don't need to come to me, Robert, for medical treatment."
"boss---"
"Don't call me boss, don't call me Mr. Robert from now on, tell everyone, from now on, call me Brother Luo, you know? Brother Luo!"
"Okay, Brother Luo!"
"Brother Barrett, you are such a smart guy. During the time I was away, I was very satisfied with your work."
"Sir---no, Brother Luo, I will continue to work hard."
"Barrett! Don't you think I am a genius? Brother Luo Clinic, this is not a name that most people can think of. In the entire United States, this must be the ceiling for clinic naming. If anyone wants to infringe, I will fight him."
"Look, Brother Luo, this is the statistics of recent Chinese learning. They are waiting for you to come back to take the exam, and then give out bonuses according to the number of words. This is Thor, the bastard. He actually mastered more than a thousand Chinese characters in a short time. He will reward you according to the number of words."
According to the rules, he has to be given one hundred thousand dollars, enough to change his car, and Jenny, she has also mastered 900 Chinese characters, and this is the number from last month." Barrett really feels sorry for his boss's money.
Robert didn't care about the money at all: "Find a time to have a formal exam, and give out bonuses based on the Chinese characters on the test paper, each worth one hundred dollars, which is a lot."
"Brinton of TIRR Memorial Hermann Hospital threatened to fire you out of office this time, saying that you are just taking the glory of your fathers. Their achievements are far above ours. They have already suffered from sports injuries of knee and shoulder joints."
Accumulated rich experience and created many advanced methods."
Barrett reports on some recent news in the industry.
The North American Academic Conference on Sports Medicine is about to be held. This conference is also a time for general elections. Robert is the current chairman, but Brinton of TIRR Memorial Hermann Hospital has always been Robert's old enemy. This person has made great achievements in sports medicine, especially this one. In the past few years, with a lot of results, he threatened to oust Robert and reorganize North American sports medicine.
In response to Barrett's words, Robert just smiled faintly: "Young man, don't be nervous, he doesn't have that ability yet. Starting from tomorrow, I will tell you Brother Luo's true level."
