Bai Ye found the scientific research process of Danshen Dropping Pills from the Internet. Because it is an old medicine for decades, many papers have been published online, and many drugs similar to Danshen Dropping Pills have been produced.
Currently, there are at least fifty or sixty kinds of liquid Chinese medicines made from Salvia miltiorrhiza and Panax notoginseng on the market. Bai Ye gritted his teeth and paid for each one.
After continuous separation and purification, Bai Ye was surprised to find that the specific pharmaceutical ingredients contained in these different preparations were actually different.
For example, Compound Danshen Dropping Pills use salvianoside and total notoginseng saponins, but there is a kind of Danshen Tablets that uses cryptotanshinone, salvia miltiorrhiza lipid 2a and notoginseng saponins. Bai Ye is a little curious, what is the difference?
Because they are common drugs currently on the market and can withstand clinical verification, Bai Ye began the process of frantically searching for information.
Bai Ye suddenly understood Mr. Liu's good intentions. It has been decades since the research and development of an old drug. He has studied it very thoroughly. He has tried it from many angles and tried every molecule or compound discovered.
Do experiments to study its efficacy and toxicity.
Liu Lao means that Bai Ye can be exercised in this process. He believes that Bai Ye is capable of discovering stable artemisinin complexes in artemisinin. Such a person's thinking ability and innovation ability are worthy of the test.
A test.
Mr. Liu asked Bai Ye to make Danqi Huoxue Pills, hoping that Bai Ye could discover the differences between these drugs on the market, why there are these differences, why some molecules should be removed, and why some compounds should be retained. These are all in the materials.
Many can be found in the database.
After Bai Ye studies this drug, his level can be improved a lot. At least for these two drugs, Bai Ye can understand them thoroughly!
If you take a medicine thoroughly, you can eat it all over the world. Bai Ye's mentor, Master Zhang Xuewen, is nicknamed Zhang Danshen because he has a very high grasp of Salvia, not only from the perspective of traditional Chinese medicine, but also from modern medical research, etc.
Have your own unique point of view.
There is a saying: If a person is good at it, he should do it step by step, step by step, step by step.
If it doesn't work, just do one thing that doesn't work, and one thing that doesn't work, that doesn't work.
The same is true for medicines. Once you make one medicine and make it well, you can make other medicines and it will be good.
So Mr. Liu wanted Bai Ye to get off to a good start.
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Bai Ye has a temperament in his bones, and he must do his best if he wants to do something. If the experiment doesn't work once, he will do it twice, and he won't stop until he gets the experimental results of the drug.
A week later, Bai Ye discovered that he had spent more than 20,000 yuan on medicines, which he never expected.
Fortunately, the Dongshan Animal Breeding Base has completely stopped operating. When Bai Ye was about to pay the rent two days ago, Meng Yunxi suddenly told him that he would not have to pay the rent this month because a company was preparing to take over and the two parties were negotiating. Everything was fine.
Go smoothly, let Bai Ye finish what he is doing as soon as possible.
Nothing happened on Bai Ye's side. The two parties met and the contract was terminated.
After the contract was terminated, the knife on Bai Ye's head finally became looser, but he didn't expect that this was just the beginning, and he would spend so much money just to buy medicine for his experiment.
Bai Ye thought about the reagents used in the past two days, and still felt that there should be an explanation with the school...
After all, the medicine costs more than 20,000 yuan, so this reagent is indispensable, right?
Thinking of this, Bai Ye suddenly felt that scientific research work is really an extremely expensive thing.
What I did was the simplest work, just separation and extraction, no experiments, and I spent so much money. If I start research again...
Bai Ye suddenly didn't dare to think anymore. Scientific research without money to guarantee it was nonsense!
But let’s talk about this later, let’s wait until the experiments have results, so we started to study again.
Every night, Bai Ye watched the rising separation and extraction skill experience, which also gave him a sense of faint happiness...
It was another three days, and Bai Ye was already a little anxious. It was already mid-June, and everyone was starting to have pre-graduation carnivals and nostalgia, but Bai Ye was still trying and repeating.
Although no new substances have been discovered, Bai Ye has become very proficient in grasping these two drugs, and even the separation and extraction have reached: lv4:1024/30000.
But now Bai Ye is taking a dim view. If there is really a new discovery, it would have been discovered long ago. He is already very satisfied with having achieved this step.
It can be said that he now has a high degree of grasp of these two drugs and the Salvia miltiorrhiza and Panax notoginseng preparations on the market.
Can't we just end it here?
Submit all the current research into a report? Anyway, the progress of the current research is already very good.
During this time, Bai Ye also took out the "Traditional Chinese Medicine Processing Experience and Grade Classification" that he got through a lottery and read it.
However, Salvia miltiorrhiza and Panax notoginseng are too mature medicines. There is not much difference between what is recorded above and what is available now. The only difference is the difference in preparation experience.
In this case, since it has nothing to do with processing, then... what will happen if hellebore is added?
We all know the eighteen anti-inflammatory properties of traditional Chinese medicine, including ginseng, peony, root and veratrum!
Ginseng and Veratrum have drug toxicity and are incompatible. Bai Ye suddenly thought, since he can't find anything new, can he add Veratrum to see what the reaction is?
Thinking of this, Bai Ye first looked up the information. There was information. Many people had done this experiment, but no one really explored how the reaction occurred. They just said that it would aggravate the neurotoxic effects of Veratrum.
Bai Ye felt that this was an opportunity. Since he couldn't discover anything new, he might as well create one.
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Three days later, Bai Ye looked at the substance on the chromatograph in surprise. He was a little dumbfounded. Did it really come out?
Bai Ye reacted many common substances in ginseng with Veratrum extract and found that there was no effect at all.
So he tried adding another substance, which was also an extract of Salvia miltiorrhiza, a substance called nortanshinone-3. But after putting it in, he was surprised to find that there was a reaction!
This nortanshinone-3 acts as a catalyst, catalyzing a series of reactions between the two extracts.
Bai Ye was shocked and found out!
But when Baiye added this nortanshinone-3 to ginseng, dangshen, panax notoginseng and other extracts to react with veratrum, there was no change at all.
What does this mean?
Specific catalyst!
Special enzyme!
Bai Ye suddenly felt confused, and quickly began to inquire about the clinical research and experimental observations of nortanshinone-3. This substance does exist, but catalyst experiments are rarely done, but this substance is often tested clinically because
The content in Salvia miltiorrhiza is very small, and it only shows up when it reacts with Veratrum.
What is the significance of this thing?
This time, when Bai Ye added nortanshinone-3 and tanshinol alone to cook together, he discovered an unusual change. Nortanshinone-3 and tanshensu were missing!
It should be that I got a new substance!
Bai Ye didn't know much about pharmacy knowledge. He checked books and began to search for information frantically. He even missed the dinner before graduation.
After checking information and doing research and analysis, Bai Ye discovered a problem and he could no longer do the next work.
What to do?
cooperate!
A person's energy is too limited, so Bai Ye found Zhou Mao. After Zhou Mao learned the news, he stood up and followed Bai Ye out.
In the laboratory, the two of them stayed up all night. Zhou Mao suddenly looked at Bai Ye with a solemn expression and said:
"Do animal experiments and observe indicators!"
There are too many instruments and equipment needed to conduct animal experiments, and the current laboratory cannot meet them at all. However, Zhou Mao is the dean of the School of Traditional Chinese Medicine, so he still has this right.
Scientific research is always a waste of sleep and food.
Two weeks later, Zhou Mao and Bai Ye met each other and smiled in the laboratory!
The results came out. When these two substances are used together, a new substance can be produced, and this substance can effectively increase the activity of antioxidant enzymes and reduce free radicals!
What does this mean?
A very simple meaning is that when these two drugs are used together, they will not improve blood circulation, nor will they dilate coronary blood vessels, nor will they affect coagulation function.
It has only one simple but not simple function: scavenging oxygen free radicals.
What are oxygen free radicals? They are an oxidation product produced by the human body every day after metabolism. These substances will be produced under other circumstances: such as stress, smoking, radiation, exposure to pollutants...
Once there are too many oxygen free radicals in the body, it will damage the cell membrane, causing the cells to lose stability and unable to work normally.
For example, it affects the secretion of hormones, the conduction of electrolytes and physiological signals, etc., and can even cause some chronic diseases. It can also cause DNA damage, causing tumors, cardiovascular diseases or cell apoptosis.
What is the end result?
Accelerates aging.
Therefore, it is now believed that taking antioxidant supplements regularly can neutralize the destructive power of oxygen free radicals, protect cells from oxidative damage, and delay the aging process.
In fact, Salvia miltiorrhiza has the effect of scavenging free radicals. As early as 1996, a research study showed that the tanshinone contained in Salvia miltiorrhiza injection can scavenge oxygen free radicals caused by myocardial infarction while protecting myocardial cells from damage.
The Department of Pharmacology at the National University of Singapore School of Medicine also used scientific methods to study the efficacy of salvia miltiorrhiza made into Western medicine. It was found that through oxidase activity measurement, salvia miltiorrhiza can help increase the activity of antioxidant enzymes and reduce free radicals.
But no one has discovered the final reason!
Bai Ye and Zhou Mao looked at each other and finally got the result!
Zhou Mao looked at Bai Ye, a smile appeared on his haggard face: "You... are really a genius!"
After getting along in the past few days, the relationship between the two has become better and better. It is no longer just a relationship between teacher and student. At first, Bai Ye called him Teacher Zhou, and later, he called him Uncle Zhou.
Bai Ye smiled, scratched his messy hair, and glanced at Zhou Mao: "Uncle Zhou, you can change your car!"
Zhou Mao heard this: "You bastard! Haha... yes, change the car!"