In fact, when Bai Ye came to Naya's laboratory, his focus was not to study the modeling of renal hypertension, but to study the model of essential hypertension through the process of modeling renal hypertension.
In "Rat Raising Experience", Sakr's essential hypertension modeling model is a product of genetic engineering, and essential hypertension is achieved by modifying a certain gene sequence.
However, you cannot become a fat man in one go. You must have a solid and stable foundation, just like you have high-level martial arts secrets but no introductory mentality.
Moreover, modeling is only a method. What is really Bai Ye's hope is to improve his level of genetic engineering through the process of using genetic engineering modeling.
The level of genetic engineering is high, and it is definitely suitable in the current environment!
The key is that this kind of modeling also allows you to complete Zhang Hanlin's serial tasks, which is also a top priority.
Keao's mission has always been on Bai Ye's mind. If he wants to prove the instability of the original km mice under drugs, and change the unstable sequence of mice to discover new products, he must establish a high-level gene
Based on engineering level.
Bai Ye's joining made Dong Cunguang even more ambitious and motivated. Every day the two of them discussed with several thick books on genetics, or they did research with mouse embryonic cells.
It has to be said that Naya attaches great importance to scientific research and buys millions of equipment at any time. Dong Cunguang's laboratory is equipped with a variety of modern scientific research equipment.
Nucleic acid molecule hybridization technology, computational gel electrophoresis technology, DNA sequence analysis technology, gene site-directed mutation technology, polymerase-linked reaction technology...
These supporting books and materials are piled up in Dong Cunguang's laboratory.
Bai Ye's learning was selective, because when he first acquired the genetic engineering skills, conditions were required for activation.
Dong Cunguang was originally engaged in bioengineering and was very accomplished in memory engineering. Under his guidance, Bai Ye made rapid progress.
In a few days, Bai Ye reached level 3 in genetic disease, molecular biology, and cell biology.
And Bai Ye also heard a ding:
[Scientific Research Skill: Genetic Engineering Activation, Current Level lv3: 3900/10000.]
Bai Ye understood that the extra 3900 experiences were the result of his studies and experiments these days.
In the next few days, Dong Cunguang received an assignment notice to attend an academic forum. Bai Ye was the only one left in the laboratory.
However, under the leadership of Dong Cunguang, Bai Ye also learned a lot about the use and operation skills of these instruments.
After this period of study, Bai Ye wanted to try research on a new type of essential hypertensive mice.
What is recorded in "Rat Raising Experience" is that it is necessary to compare the molecular sequence of ordinary rats with hypertensive rats of the same species, and then obtain matching nucleic acids, and then use the shotgun method at the mutation point. In this series of processes,
It is carried out under an ultramicroscope, and the most important thing is if there is a suitable hypertensive mouse model.
Dong Cunguang has been studying the shr gene sequence for a long time. Based on his research, Bai Ye felt that the time was ripe.
Bai Ye extracted DNA sequences from embryonic cells of ordinary SD rats, analyzed them in a DNA molecular sequence analyzer, and then separated them by electrophoresis to obtain nucleic acids.
However, shotgun replacement is not that simple, and many types are very prone to problems when separated under a microscope.
However, when Dong Cunguang came back, the progress of the research was greatly advanced. However, not all recombinant DNA can survive, especially inbred mice like shr, which already has essential hypertension and various recessive diseases.
Gene, after it is combined with ordinary mice, it is not easy to form a stable nucleic acid sequence.
Bai Ye looked at the two sets of DNA sequence diagrams and said, "Actually, we may have entered a misunderstanding."
Dong Cunguang was stunned: "What do you mean?"
Bai Ye frowned and looked at the two sets of sequences: "Maybe we can change our thinking and improve shr instead of putting the shr gene into other mice."
Dong Cunguang's eyes suddenly lit up: "You mean to use the stable nucleic acid structure of ordinary mice to compensate for the genetic defect of shr?"
………………
………………
Three days later, the two have cultivated a batch of new embryonic cells, which are waiting to be implanted into female mice to see the results after hatching.
Waiting is the longest process, but Bai Ye has gained a lot.
[Ding! The most expensive member, your scientific research skills and genetic engineering level will be upgraded to lv4.]
After reaching level 4, Bai Ye felt that many of the originally complex problems in his mind suddenly became clear. This was not only an improvement in level, but also a systematization and perfection of knowledge.
Bai Ye felt that the originally hazy and mysterious DNA sequence seemed to have a clue.
At this time, the new model of mice needed to wait for the results, but Bai Ye did not want to continue the research.
He has a more important thing to do, which is research on the stability of the km mouse gene.
With the foundation gained during this period, Bai Ye feels confident.
However, as the km mouse is a mature and commonly used experimental mouse, it is obviously an unwise idea to deny its stability.
However, after Bai Ye reviewed a large amount of data, he found that there were indeed some examples of experimental failures, and the reasons were unknown. However, many of the animal experiments used in these experiments were km mice.
In fact, why mice are used as experimental animals for human drugs is because researchers have analyzed 96% of the mouse genome sequences, and 99% of the genes can find homologous sequences in the human genome sequence.
It was confirmed that mice and humans are highly homologous at the genetic level.
But it doesn't mean that mouse and human genes are similar.
90% of the numbers in sequence a can be found in sequence b. Do you dare to say that the two sequence are very similar?
In fact, the relationship between these two series is similar to the relationship between the mouse genome and the human genome.
99% of mouse genes can find homologous sequences in humans, but the human genome cannot be found in mice.
What Bai Ye wants to do is to prove the instability of the a sequence and prove that the special genetic structure of mice will, under certain conditions, allow mice to adapt to the environment and slightly change their own genetic sequence, and the changes will not
Not suitable for drug testing on humans.
How to find the cause of mouse gene instability is what Bai Ye needs to consider now.
ps: The number of free words, the recent additions are very poor, veterans once felt like they were collapsed and panicked.
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