With the previous foundation, Bai Ye is now able to handle mouse genes with ease.
The study of gene stability in km mice had to find a starting point. After searching countless data, Bai Ye found the drugs that had been used in failed experiments. Perhaps these would be an opportunity.
It's just that those drugs are really hard to find. Bai Ye spent a lot of effort to find the data of those failed experiments.
The next step is to stimulate various experiments, hoping to find variant RNA sequences, transcription and translation that may be the cause of the changes.
However, there was little progress in the subsequent research. Bai Ye could hardly find any points of mutation. These genes were very stable.
Shouldn't it be studied in genetic status?
Will simply studying a genetic sequence and studying it in mice have any undesirable effects?
During lunch, Dong Cunguang found Bai Ye.
"There is an academic meeting at the Hyatt Hotel tomorrow, I suggest you take a look."
Bai Ye was thinking about mice while eating and sleeping. Hearing Dong Cunguang's words, he raised his eyes and asked, "What is the meeting about?"
Dong Cunguang mixed his rice: "It was a meeting held by Lonca to promote SHR and its model mice. Many experts were invited, as well as staff from European gene banks. I think you can go and listen to it."
The experiment had come to a standstill, but Bai Ye still felt the need to listen, hoping to gain something.
The next morning, the two went to the Hyatt Regency Hotel, paid 2,000 yuan, and got two passes to attend the conference.
The lecture was held in a large conference exhibition hall. Bai Ye and Bai Ye chose a good place in order to hear clearly.
The speaker on the first day of the conference was a domestic master in the field of genetic engineering: Zhang Shulan, a doctoral supervisor in medical bioengineering, graduated from Johns Hopkins University with undergraduate and graduate degrees. She originally had the opportunity to stay in school and resolutely returned to China and entered China.
Academy of Sciences, working in the field of bioengineering.
Zhang Shulan is good at gene splicing technology. The main content today is how to use molecular genetics as the theoretical basis and modern methods of molecular biology and microbiology as means to realize genes from different sources according to the pre-designed blueprint.
Hybrid DNA molecules are constructed in vitro and then introduced into living cells to change the original genetic characteristics of organisms and obtain new varieties.
This is the use of modern means to break reproductive isolation and achieve the development of genetic engineering.
Zhang Shulan spoke very seriously, especially focusing on the basic theories and the exploration of new ideas.
What Zhang Shulan talked about was very basic, and there was not much advanced and cutting-edge knowledge. However, this kind of conference was not a professional academic conference, but a marketing promotion of one's own products in the name of an academic conference.
But Bai Ye also gained a lot. In two days, these experts and professors systematically sorted out the knowledge, which further consolidated Bai Ye's genetics, which was not solid at first.
On the third day of the conference, which was the last day, it seemed that several internationally influential big names came to the venue.
"Let us give warm applause to Dr. Macalette from the European Gene Analysis Library. Dr. Macalette has been engaged in mouse genetic analysis for decades and has unique insights into mouse research..."
Dong Cunguang whispered to Bai Ye: "Makarat is one of the main experts who conducted the shr research at the beginning. His analysis of the shr gene is very good and has basically been completely analyzed..."
Bai Ye nodded: "Then he didn't come up with the idea of improving SHR?"
Dong Cunguang sneered: "When thoughts are shrouded in gold coins, knowledge begins to be biased."
"And what Macaret is best at doing research is to prove the advantages of shr. He believes that shr mice are the product of God because they have been cultivated for hundreds of generations. Of course, the most important thing is that he has done experiments on shr many times.
When the high blood pressure gene fragment is implanted into other mice, it is difficult for those mice to show the universal and stable high blood pressure like shr. Of course, I hope to remain skeptical."
"However...gene mutations are uncertain, including gene grafting. The resulting mice will produce different results based on individual differences, so they are not suitable for mass production and experiments."
Makalite is mainly talking about shr (essential hypertension) mice today. I have to say that he is very professional and has made a lot of efforts in the field of continuous development and innovation of shr.
"Biological evolution is the most perfect genetic engineering. It can get the most suitable genes to change their biological attributes. After hundreds of generations of research, shr, we have summarized the shortcomings of each time and hope to change it from the perspective of genetic engineering. Of course,
, this is a process, just like bioengineering is a developing discipline, we can only change if we continue to understand new things."
…………
Finally, Macalette asked, "Do you have any questions?"
Everyone smiled and shook their heads, but at this time, Bai Ye stood up: "I would like to ask, why were the mice selected for the first generation wistar mice instead of km mice or guinea pigs?"
Everyone turned to look at Bai Ye. Some were surprised, some were curious, some were dismissive, but some were thinking about this issue.
Makalite was stunned and looked at the person who asked the question with interest. He had held more than one meeting in Kyoto, but he was still the first person to ask this question: "This is an interesting question. In fact, I have also used it.
The effects obtained by other mice are not as good as Wistar. Generally speaking, the homologous genes of these mice are the same, and many mice are even obtained from hybrid genes. But here it involves gene stability and recessive genes.
Expressive questions.”
Bai Ye's eyes suddenly lit up. This was the first time he heard about the issue of genetic stability.
Makalite continued: "It is well known that the gene stability of km mice is the strongest, and the genetic structure is not easy to mutate. However, obtaining mice with similar high blood pressure may require more energy and time to cultivate, and it may not necessarily be obtained.
Plant 100% high blood pressure.”
"But during the experiment, it was found that the gene stability of this wistar mouse is strong, and the recessive gene expression is very good... I think this may be the reason."
Bai Ye kept digesting Makalite's words in his mind, and suddenly an idea came to his mind: "Wistar mice and km mice are both very stable mice, and km mice are also inbred mice. It stands to reason that they can last for countless generations."
After breeding, recessive gene expression should appear, but why can't it be found? Is it because of adaptability? "
Bai Ye then asked: "The gene sequences of wistar mice and km are not much different. Isn't the gene expression you said contradictory? If it is really like what you said, I would like to ask about the adaptability and adaptability of mice.
Is the strong survivability related to the selective expression of genes?"
As soon as Bai Ye finished speaking, there was an uproar on the scene.