Cai Zixuan looked left and right before saying, "The leader of our group is Wang Ruoxiang."
"Is this the case?" Gu Jun frowned helplessly, thinking it was something. "It's not surprising at all, okay. I'm surprised if it's not her."
"Yes, that's Wang Ruoxiang." Cai Zixuan was filled with emotion when he heard this.
Wang Ruoxiang is the monitor of their class. She is very capable, beautiful, and has a good personality. For such a top-notch academic goddess, those who pursue her are of course lined up from the teaching building to the bureau. Jun is one of them, and the pursuit was so vigorous back then.
, but in the end it ended with Wang Ruoxiang sternly refusing in public: "I'm sorry, we are not the same kind of people. I won't like people like you."
Uh-huh, a tall, handsome, rich man who still has an eight-year job no matter how bad he is, is actually "this kind of person"?
At that time, everyone said that they were grateful to Tuhao Jun and the squad leader for making them believe in love again.
"Monitor is still single now." Cai Zixuan obviously doesn't know how much of a bitch he looks now.
"Don't look at me like that." Gu Jun laughed at himself, "It's all over." Indeed, it's all over. The local tycoon Jun pursues the squad leader partly because he likes it, but partly because he wants to chase everyone's goddess. In the final analysis, it's all over.
Those profound things don't matter.
"Really?" Cai Zixuan stopped talking about the squad leader and looked at his watch, "Hey, it's almost 8 o'clock. I have to go to the laboratory."
"Let's go, I also want to go to the experimental building." Gu Jun said.
Cai Zixuan suddenly showed a knowing look in his eyes. Just now he said he didn't care about the squad leader, but now he turned around and joined in the fun. It's great to be young.
"What?" Gu Jun was really helpless. He wanted to explain but didn't know where to start, "Forget it..."
"Hao Jun, you don't have to say it, you understand. It was once difficult to make water in the sea, I understand."
"You know what a sponge is."
The Medical Laboratory Building is located in the northwest of the campus. It is a dozen-story white brick building with no features on the outside. Even opposite the door is a rotten cement floor that has been in disrepair. But inside the building, there are laboratories with various equipment and equipment.
Teachers and students use it for scientific research.
"This way." After entering the building, Cai Zixuan walked in front, introducing the situation to Gu Jun as he walked.
The title of the experiment conducted by their group is "Research on Photodynamic Treatment of Tumors with Several Photosensitizers". It is a study by subcutaneously implanting tumors in mice, then using different photosensitizers for photodynamic treatment, and then observing the changes in the tumors.
.
The funds allocated this time are only 30,000 yuan, which is a lot for an undergraduate scientific research experiment, but an imported pure photofrin photosensitizer costs tens of thousands of yuan. In order to make more photosensitizers, Wang Ruoxiang made various
We budgeted carefully and saved money to set up a domestic imitation photofrin group, a dotiporfin group, an ala group, and a blank control group, a total of 5 groups of mice.
"The research plan and the proposal report were all done by her, the team leader." Cai Zixuan couldn't help but sigh when he talked about it, "She really deserves to be Wang Ruoxiang."
Although Professor Gu is a tutor, he only takes the time to give guidance occasionally. For a great guy like him, it is his business to achieve high scores in SCI articles. Therefore, the Pioneer Cup still depends on the students themselves. The team leader does the most, but it is
The opportunity that everyone dreams of.
Because if the experiment produces a paper, Wang Ruoxiang will be the first author, and Cai Zixuan and the others will have to be ranked behind.
"We are still in the first stage, which is to establish an ascites-type tumor-bearing mouse model. We have been cultivating it for a week, and we can't think of it today."
Cai Zixuan talked for a long time, and suddenly found that Gu Jun had not spoken for a while, and seemed to frown when he heard the word tumor...
"Uh Haojun...you know, the success rate of modeling is not 100%."
Cai Zixuan scratched the bald part of his head. He really didn't know if Gu Jun understood what he just said.
"There is nothing in this world that can be 100% perfect." Gu Jun lowered his head and covered his face. My friend, I haven't learned to be a scumbag yet, okay?
"Indeed." Cai Zixuan was deeply touched by these words, and his face was filled with vicissitudes of life. "All dharma is like a dream."
Okay, Gu Jun doesn’t understand now…
During the conversation, the two took the elevator to the eighth floor, where the laboratory used by their team was located.
Walking along the corridor and reaching the door of the laboratory, Cai Zixuan opened the door and walked in first. He saw that all the team members had arrived, and he was the last one.
"Zixuan, I'm just waiting for you."
"Huh?"
At this time, everyone saw a person walking in, and the laboratory suddenly became silent, with only the chirping of mice remaining.
Rich guy...?
Didn't it mean that he dropped out of school to travel around the world? Didn't he say that he failed? Didn't he say that he...
"Hello everyone." Gu Jun waved to everyone with a smile and looked at Wang Ruoxiang, "Hello, squad leader."
Wang Ruoxiang was standing next to an experimental table. She had medium to short black hair, a delicate oval face that was very good-looking even though she was bare-faced, and a tall figure wearing a white coat. She was full of heroic spirit. Wang Ruoxiang frowned slightly when she saw him.
, "Hello, Gu Jun." The voice is also very nice.
Gu Jun nodded in response to her. After three months of separation, the squad leader was still so beautiful, but he was no longer the same person as before.
"Hao Jun had to take a leave of absence from school before, and now he is back." Cai Zixuan explained to everyone, and introduced to Gu Jun: Xu Hai, a classmate in the clinical major, is an acquaintance, as well as Zhang Haoran in the basic medicine major, He Yuhan in the pharmacy major, and a group of 5
Everyone is here.
Cai Zixuan asked: "Squad leader, Hao Jun wants to take a look with us, is it okay?"
Wang Ruoxiang pursed her lips silently and then said: "Just stand aside and don't cause trouble."
Rich man. She murmured in her heart, remembering that she had a superficial liking for him at the beginning of school a few years ago... ugh. This kind of person is lazy and lazy, full of all five poisons, rotten wood cannot be carved, and mud cannot hold up the wall... Xiao
Not as good as a rat! Maybe you can only make a contribution to medicine if you are pulled into slices for study.
"No." Gu Jun stood aside obediently, but he always felt that Wang Ruoxiang's eyes were not kind...it was an illusion.
There are already a row of mouse cages on the experimental table. Each cage contains a group of white Kunming mice. These are the most commonly used experimental animals. They are very docile and compact, weighing only 1-1.5 months old.
18-22g, body length less than 10cm, ready for experiment.
Except for one cage of modeling mice, the others are all blank mice, which Wang Ruoxiang just bought from the school's experimental animal center. Once the modeling is successful, they can be put to use.
Now that the team members have arrived, everyone starts working immediately.
Crack! Wang Ruoxiang put on rubber gloves and went to the modeling mouse cage to catch mice. These mice were all marked with numbers using dyeing methods. She caught mouse No. 1. Cai Zixuan and several others were assisting and recording
, video and photography work, etc.
"On the eighth day of intraperitoneal implantation of S180 mouse fibrosarcoma." Wang Ruoxiang looked focused and serious. She gently pressed her hand on the obviously bulging abdomen of mouse No. 1, and the mouse let out a painful cry.
He struggled endlessly, but was firmly held in her hand.
She said: "The ascites of mouse No. 1 continues to grow, and it is already the belly of a frog. It has good vitality and does not need to be killed for the time being."
Hearing what she said, the team members suddenly became excited, and they all moved closer to get a closer look, "Is this the 'frog belly'?" "Awesome."
"As expected of a frog's belly, it's so big." Cai Zixuan said with emotion.
Although they are all in the eight-year program, this is actually their first time doing such a real scientific research experiment.
"Oh." Gu Jun also came over to take a look, and he still understood what they were doing.
The reason why ascites tumor-bearing mice are first created is to obtain a large number of highly active S180 tumor cells. You must know that the original cell lines are very expensive, one tube is only enough to inject one mouse, and the activity is very fragile.
, and the four treatment groups they set up required cultivating hundreds of mice.
So it is created first and then expanded. Now the mouse has grown a frog belly and can "retract abdominal fluid".
"Gu Jun." Wang Ruoxiang suddenly turned her head and glanced at him, "Can you step away? I'm in the way."
"Okay." Gu Jun shrugged and walked away. You have a good aura and can be in your way even one meter away.
Cai Zixuan and the others pretended not to see it, and Wang Ruoxiang continued with the next step.
She inserted a 5ml syringe into the abdominal cavity of mouse No. 1, successfully drew out a tube full of yellow clear ascites, and then injected it into an eppendorf conical bottom centrifuge tube. She then used a centrifuge for centrifugation. Once centrifuged,
Immediately you can see a large amount of white precipitate appearing at the bottom of the centrifuge tube, which is tumor cells.
"Mouse No. 1 was successfully modeled." Wang Ruoxiang was relieved and happy, and smiled to the team members: "There are a large number of S180 tumor cells in the ascites!"
"Yeah!" "Haha." They were all young people, and no one paid attention to the mouse's feelings. They cheered, and Cai Zixuan praised: "This is the first step to success!"
The first step was successful, and then the cells were given to the mice in the treatment group to create subcutaneous tumors. After the tumors were formed, photodynamic therapy was performed.
There was still a lot to do, and they continued to work, removing the supernatant from the centrifuge tubes, resuspending and counting, adjusting the cell concentration...
In order to maintain cell viability, all injections must be completed within one hour. So they finished one modeling mouse and then moved on to the next.
"Adjust the cell concentration to 1x10^7/ml..."
"Yuhan, you are responsible for taking 0.2 ml of cells per injection."
"Team leader, the anesthetic is ready!"
Everyone of them was so high-spirited that no one cared about what happened to Tuhao Jun, and even forgot that he was standing aside and watching.
Gu Jun watched quietly by himself, watching He Yuhan prepare the cell suspension, Cai Zixuan anesthetized the healthy mice with intraperitoneal injection of sodium pentobarbital, Xu Hai and Zhang Haoran then marked the mice with numbers and removed the hair on the buttocks, and finally Wang Ruoxiang
Mice were injected with tumor cells subcutaneously in their right buttocks...
No. 1, No. 2, No. 3, one after another.
Suddenly, Gu Jun's head felt a splitting pain. Could it be a brainstem tumor attack...
The pain suddenly intensified, and he couldn't help but cry out in pain: "Ah!" The scene in front of him became blurry, flickering like the shadow of a nightmare.
He seemed to be unable to breathe air, as if he was in a ruined laboratory, with centrifuges, microscopes, sterilization pots and other instruments lying on the floor, broken test tubes, messy documents, blurred picture frames, and putrid animals.
Corpses...the dust turned everything into a gray color, without any breath of life, only chaos and deathly silence.
The pain in his brain became more and more severe, but the scene became clearer. He could clearly see some unspeakable things.
There are also those twisted corpses on the ground...it is impossible to identify what kind of animals they are...
He saw again that there was a line of blood written on the wall inside. It was the mysterious language on the medicine box again, but compared to the standard of printing, every stroke of the line of blood had a creepy feel.
weird.
In a daze, Gu Jun suddenly seemed to be able to understand the meaning of the bloody words.