Chapter 1067: Take only one scoop out of three thousand weak water
Sisi's body recovered well. After evaluation, Yang Ping felt that it was possible to use the virus without castration for experiments. Although there were certain risks, he decided to give it a try.
If you always stand still and don't catch risks, it will be difficult to make some breakthroughs. Taking risks is feasible if you are fully evaluated and prepared.
Even if it fails, it will be treated as a serious cold, and Yang Ping is actively preparing to continue the experiment.
The laboratory of Nandu Medical University has begun a new round of various tests on the K virus. After multiple repeated tests, the new K virus is considered to have not deviated from the basic characteristics of the source virus. The safety of experiments using viruses that can replicate can be obtained.
ensure.
The oncology laboratory here at Sanbo Hospital has also concluded through a series of examinations that the K virus currently infects most tumor cells and a small number of normal cells. However, from the observation of several experiments, it can only cause tumor cells
Apoptosis will not cause apoptosis of normal cells. Its impact on normal cells is lower than the impact of its source virus, adenovirus, on human cells.
The vector source virus used by K virus is an adenovirus---but it is not an ordinary adenovirus, but a recombinant adenovirus. Recombinant adenovirus, this replication-deficient adenovirus vector system, is basically used in gene therapy.
It is widely used in life science research and other fields.
Recombinant adenovirus has the following significant advantages: it has a wide range of infection and can infect almost all cell lines, primary cells and some tissues; its infection efficiency is as high as 100%, surpassing other viral vector tools and liposome transfection;
It has a large carrying capacity for foreign genes and can be as high as 8Kb; it does not integrate the genome; it has high titer and is easy to operate. Therefore, recombinant adenovirus has always been considered the most potential gene delivery tool.
Of course, Yang Ping borrowed it not to deliver genes, but to deliver K factor. Before using it, Yang Ping further improved it. Then he implanted K factor into its protein structure to form
A protein complex.
The currently commonly used adenovirus vector is based on human adenovirus type 5, whose genome is a 36Kb long linear double-stranded DNA. This adenovirus is endocytosed into cells by binding to its own fibers and receptors (such as CAR) on the cell surface.
, and then transferred from the endosome to the cytoplasm and nucleus, using the cell's transcription and translation machinery to initiate virus replication and assembly. A complete virus life cycle will trigger cell death and release virus particles.
Ordinary adenovirus is not a good thing. Once infected, this virus can easily cause diseases in the respiratory tract, digestive tract, urinary system and other parts of the body, such as pharyngitis, pneumonia, conjunctivitis, etc., causing fever, cough and phlegm, and pharyngeal redness and swelling.
Symptoms such as congestion, diarrhea, vomiting, etc. can even cause difficulty breathing and suffocation in severe cases.
But the recombinant adenovirus is completely different from the ordinary adenovirus. The difference between them is similar to that of a dog and a wolf. The recombinant adenovirus has been completely tamed by researchers. Not only has its replication gene been knocked out, but its genes and genes are still being mastered.
On the basis of its characteristics, some of its pathogenic genes have also been knocked out. Therefore, it is often
The recombinant virus no longer has strong pathogenicity, but retains some other characteristics useful for experiments, such as: wide infection range, high infection efficiency, and after Yang Ping's transformation, it is effective against incompletely differentiated tumors.
Cell infection is very high, and the infection rate of normal cells is lower than that of ordinary viruses.
Therefore, the adenovirus that has been carefully recombined by Yang Ping has great potential. It is a major innovation in itself. It can be used not only in K virus therapy, but also in many future tumor treatments. It is equivalent to the treatment of tumors.
A high-energy, high-precision weapon has been added to the arsenal.
Despite this, Yang Ping did not dare to take it lightly. He carefully re-examined the genes of these viruses and confirmed that there were no problems with their genomes.
This recombinant special adenovirus has a huge advantage over the original ordinary adenovirus.
The original ordinary adenovirus is highly contagious because it can survive for 30 minutes in an environment of 56°C and two minutes in an environment of 100°C, which provides conditions for its infectivity.
The adenovirus recombined by Yang Ping's team can survive for no more than one minute in a dry environment, and the average survival time is just over 20 seconds. Even in a humid environment, the survival time at a temperature of 56°C is not long.
More than one minute. Therefore, this virus is extremely fragile. It cannot survive in an in vitro environment. It can only exist inside the human body. Moreover, the human immune system maintains sufficient lethality against it, which makes it far more threatening to the human body.
Much lower than ordinary adenovirus.
So no matter how you look at it, this recombinant adenovirus is very safe, so Yang Ping's adventure is not random, he only does it when he is sure enough.
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The K-virus cultured in the virology laboratory of Nandu Medical University has been encapsulated and sent to Sanbo Hospital via cold chain transportation. Yang Ping injected the viruses into Sisi's body.
Obviously, the live virus has a much greater impact on the human body than the castrated virus. On the third day after the virus was injected, Sisi had a high fever again, but this time the body temperature was not that exaggerated, only 39 degrees. For someone who had experienced 42 degrees many times,
Sisi was tested by a high fever, and he could definitely withstand a 39-degree fever.
This time the fever lasted longer than before, but fortunately, it kept fluctuating around 39.0 degrees. The highest point did not break through 40 degrees, and the lowest point was about 38.5 degrees. Its heat pattern was wavy, which
It is consistent with the K virus fighting the immune system in the body
The situation is because the current K virus is completely different from the previous K virus. The previous K virus knocked out the replication gene. It is a castration virus. Under the attack of the immune system, its number will only continue to decrease, so
Once a high fever passes its peak, it can only go downhill.
This chapter is not over yet, please click on the next page to continue reading! The current K-virus has the ability to replicate. After the human immune system kills part of the virus, it will restore the number of viruses through replication. In this way, the virus and the human immune system are in a tug of war.
War, this kind of tug-of-war manifests itself in a wave shape in the form of heat.
The tumor biopsy specimens sent for examination through puncture were indeed in line with Yang Ping's speculation. It was obviously different from the previous experiments. The rate of virus infection of tumor cells in this experiment was very high. Observation of the tumor cell specimens obtained from the puncture showed that it actually exceeded 10% of the tumor cells.
are infected, and this rate is still rising with the replication of the virus. The specimens taken out by puncture at several different locations are extremely balanced.
In view of its dangerous nature, intracranial tumors cannot be punctured to collect specimens. However, judging from the infection rate of tumor tissue specimens in other parts of the body, it is estimated that the possibility of intracranial tumors being infected by viruses is almost the same, because the modified virus can
It is able to penetrate the blood-brain barrier.
This result made Yang Ping very excited. It showed that after being attacked by the human immune system, part of the K virus successfully reached the tumor cells. After reaching its destination, the K virus began to use the nutrients of the tumor to replicate.
I didn’t expect that a cautious risk would yield such great rewards. It seems that stability may not always be a good thing. Innovation requires a spirit of adventure.
With continued observation, these infected tumor cells have now begun to undergo apoptosis in large areas. Judging from the calculated ratio, almost more than 3% of the tumors have died. The infection rate and apoptosis ratio remain the same as before.
A ratio of three to one. Because the increase in infection rate directly leads to an increase in the apoptosis rate.
What is the concept of an apoptosis rate of 3%? This must exceed the growth rate of the tumor. If the treatment continues like this, there is hope that the tumor can be cured. I did not expect that replacing strict viruses with live viruses would actually bring such good results.
At this point in the experiment, Yang Ping also began to think about a name for this K virus. In fact, after many modifications, it can no longer become a virus. It must be given a brand new name---targeted apoptosis of incompletely differentiated cells.
Protein complex factor, Yang Ping wrote this name on the whiteboard in his office.
Looking at this lofty name, Yang Ping leaned on the back of the chair in his office to rest. He hasn't gone back these days. Now he should go back and hug his son. Of course, he should also hug his wife.
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Sisi's heart examination data showed improvement every time, indicating that the tumor continues to shrink. The improvement in heart function means that the impact of intracranial tumors on the brainstem is reducing. This visible sign
Changes are more reliable than experimental data. After all, any data in clinical medicine must be converted into clinical changes to have practical significance.
After the virus injection this time, except for fever for a few days, no other adverse symptoms appeared, which once again increased the confidence of the experimental team.
Recalling the stumbles at the beginning of the previous experiment, and now everything is under control, everyone couldn't help but feel very emotional. Professor Yang is still the most powerful, his head is like an alien.
K factor, recombinant adenovirus, and the combination of the two can be directly used in clinical experiments, using replicable viruses for experiments, and classifying this virus to eliminate it from viruses. It is believed that it should be replicable with K factor and be able to initiate tumors.
The protein complex of the cell apoptosis program is not an astonishing invention.
The beginning of his experiment seemed crude and completely fanciful. It felt so unreasonable that researchers at the Virology Laboratory of Nandu Medical University at the time even privately discussed that this idea could not be realized because it was so unreasonable.
However, as the experiment progressed, it became clearer and more likely to be realized. Slowly, it succeeded. The whole process was a bit incredible.
Moreover, Yang Ping's calmness and rationality towards failure surprised them, because most people are somewhat affected by failure, but he was not affected at all. He was still full of passion after failure, as if he had already determined that he would succeed.
Yang Ping himself doesn't know why he has such a talent. He can always maintain strong passion and curiosity, and accurately grasp the research direction by combining intuition and logic. Perhaps it is the result of long-term devilish training in the system space.
Sisi's magnetic resonance image came out again, and Yang Ping measured the tumor volume. Especially the intracranial tumor, not only did it not continue to increase, but it also decreased compared to before. Although the decrease was very small, it was less than the previous measurement.
The amplitude has increased a lot.
The data is consistent with the clinical symptoms, and now we can freely conduct subsequent experiments. As for ethical issues, there is actually a logic of winners and losers. When you fail, you will attract countless accusations. When you succeed, others will only
Seeing your results will make you forget your process.
During the rounds, Yang Ping specifically noticed the cactus in the corner. It was getting greener and stronger, and Sisi's energy was much better than before.
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Masao Fujiwara had a certain friendship with Director Han before. He wrote a letter to Director Han, hoping to help arrange for Miyuki Fujiwara to come to China for long-term further study, which would last for about three years.
Miyuki had a short-term training here before and felt very good, so now she wants to have a longer training, hoping to learn Professor Yang's superb medical skills and contribute to the health of Japanese people in the future.
After reading the letter, Director Han thought in his mind that Miyuki Fujiwara met the conditions regardless of her Chinese proficiency or other aspects. International mutual learning is a normal exchange.
But now, Director Han is not very familiar with the situation in Yang Ping. Yang Ping has long been the subject leader of an independent research institute, and his current job is just to provide logistical services. Director Han told this matter
Yang Ping, let Yang Ping decide for himself.
Yang Ping thought for a while, and those who came to study at this time, whether domestic or foreign, must be treated with caution. He forwarded the matter to Manager Qiu.
Manager Qiu is currently in charge of the entire security work around Yang Ping. Manager Qiu is extremely vigilant about Miyuki Fujiwara's visit. He thinks it may be related to Yang Ping's latest research results because the Huang family has told him in advance.
The importance of Professor Yang’s research results may shake the fundamental interests of many pharmaceutical giants. These people will either try to recruit
To recruit Yang Ping, or go out of their way to steal the research results. If they are unable to do the first two, they may take desperate measures and use extreme measures to stop Yang Ping's research. When bloodthirsty capital goes crazy, it is possible to do anything. Manager Qiu will
The matter was immediately reported to the superior, who replied: She can be allowed to come, but she must be made good use of to grasp the opponent's deployment as soon as possible.
Before coming, Miyuki Fujiwara contacted Xiao Su and Tang Fei. When she was studying in China, the three of them had a very good relationship and were good sisters. They once traveled to Hangzhou together and this time they came back to say hello to the good sisters in advance.
Regarding Miyuki's return, Yang Ping had no trouble in his heart. On the issue of men and women, his idea was very simple - he would only take a scoop out of 3,000 yuan. He now has a wife and children, and it is impossible to make mistakes in this regard.
.
So whether she comes or not, Yang Ping doesn't have much idea. Whether he agrees to her coming or not is left to Manager Qiu to decide.