After Jiao Zhengde learned that Bai Ye wanted a model of hypertension, he called a man. Ten minutes later, a man with glasses in his thirties walked in.
"Teacher, what do you want me to do?" the man asked.
Jiao Zhengde introduced: "This is Bai Ye. He is going to learn modeling here during this period. You can take him and study hard."
"This is Dong Cunguang, my student. He has made some achievements in the field of mouse modeling research. If you have any questions, feel free to ask."
Bai Ye's laboratory was arranged next to Dong Cunguang's, so that he could take care of anything.
Dong Cunguang is a relatively orthodox person. He wears glasses that are upright. Although he is unsmiling to Bai Ye, he always responds to Bai Ye's requests.
In the days that followed, Bai Ye did not make models by himself, but studied with Dong Cunguang and then read books in private.
What Zhang Hanlin needs are two kinds of mice, one is essential hypertension and the other is renal hypertension. His main research is the clinical research efficacy of essential hypertension and secondary renal hypertension.
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Dong Cunguang was a cold-hearted man, perhaps not cold, but unsociable. It was only during the time when Bai Ye came into contact with him that he realized how extraordinary he was.
The 31-year-old has published 8 SCI papers. His project: Improved modeling of renal hypertension in rats won the second prize of the Kyoto City Youth Scientific Research Progress Award. Originally, Dong Cunguang had already been granted a PhD.
However, Dong Cunguang is the eldest of four children in his family, and he can no longer afford to study.
It happened that Tang Ya offered him an annual salary of one million at that time, and Dong Cunguang joined the company without any hesitation. However, the money was not wasted. He successively improved several modeling methods, which brought a lot of profits to the company.
In the past few days, Dong Cunguang was still modeling rats and observing changes in blood pressure. This had become his daily life, and the two of them gradually became familiar with each other.
Bai Ye would go early every day to prepare the laboratory, and even take the initiative to take on some tasks within his ability.
Dong Cunguang also gradually began to communicate more: "How about you try it yourself?"
Bai Ye was stunned and nodded happily. After a few days of observation, he was already eager to take action.
Dong Cunguang stared at Bai Ye and said: "I have been making a model of renal hypertension these days. You probably have an impression of it. You can try it first today and I will point out any problems to you."
Bai Ye found a relatively large male SD rat, weighing about 180g. He first used scissors to remove the hair, and then disinfected it with iodophor.
Renal hypertension modeling in mice is actually very simple. In fact, the renal artery is clamped with an artery clamp. The gold-blatt hypertension model has double kidney and single kidney models. Double kidney means retaining both kidneys, but giving one or two kidneys.
Renal artery stenosis is also called double-kidney clamping or double-kidney double clamping. The single-kidney model involves removing one kidney and preserving the renal artery for stenosis, which is one-kidney clamping.
Two kidneys in one clamp is currently the most widely used, classic, and highly recognized method of creating hypertension models in the world. It is simple to create, has a high success rate, and has a low mortality rate.
Bai Ye made a 1cm longitudinal incision on the left side of the center of the rat's abdomen, with a length of 3-4cm. He used 10% chloral hydrate for anesthesia, 1ml for 180g body weight, and intraperitoneal administration...
But what makes Bai Ye embarrassed is that the mouse died...
Dong Cunguang looked at the embarrassed Bai Ye and said quietly: "He is already dead, no need to cut anymore."
Bai Ye didn't think anything of it at first, but after listening to Dong Cunguang's words, he actually felt guilty about whipping a corpse...
Dong Cunguang said calmly: "This kind of modeling has a high success rate and a very low mortality rate, less than ten percent. The most likely problem is anesthesia. When you are anesthetized, if I guessed correctly, where you injected
Probably the heart."
Bai Ye: "..."
………………
However, everything is difficult at the beginning. After a few days of study, Bai Ye was gradually able to complete the mouse modeling independently, and the success rate was very high.
In ten days, Bai Ye made nearly 100, while Dong Cunguang made 500. This was just what he made casually...
These days, Bai Ye and Dong Cunguang have gradually become familiar with each other.
Bai Ye thought of something and asked, "What do you think about essential hypertension modeling?"
Dong Cunguang pondered for a moment and stopped what he was doing: "It's difficult to do, mainly because the results lack uniformity and there are large individual differences. The cause of essential hypertension is unknown, so there are many methods in the modeling process.
Such as neurological, spontaneous hypertension, etc."
When Bai Ye heard this, he suddenly thought that the new model in the mouse-raising experience required the hypertensive mouse gene, so he asked: "Is there a mouse with the hypertensive gene?"
"Yes! There must be some. The most commonly used one in the world is shr. Dr. Okamato mated male wistar rats with blood pressure of 19.3~23.3kpa and female rats of the same species with blood pressure of 17.3~18.6kpa. Their offspring were selected
Those with high blood pressure exceeding 24kpa were called spontaneously hypertensive mice (shr). Through continuous breeding, in 1969, the inbred strain shr was obtained. By 1986, this strain had been passed down to the 80th generation. And technology
It’s very mature.”
Bai Ye's eyes suddenly lit up, because according to his experience in raising mice, mice with essential hypertension need to be genetically replaced with mice with hypertension genes to model essential hypertension. Therefore, after learning about shr mice, Bai Ye was also happy: "Then
What proportion of them have high blood pressure?"
Dong Cunguang: "100% hypertension. Generally, blood pressure will increase with age after birth. According to statistics, in the 30th to 32nd generations, the average blood pressure of male rats in the tenth week is 24.5±2.3kpa, and that of female rats is about 23.7±1.9kpa.
It will continue to rise from now on.”
Speaking of this, Dong Cunguang shook his head: "The price of an SHR is about 500 in China, but the SHR made in China is very ineffective and has a very high prevalence rate. In comparison, the overseas ones are more stable and the price is also low."
It’s only 200-300, so this kind of mouse is very valuable. Our company also did training at the beginning, but unfortunately the core technology of SHR training cannot be passed on. There is a big gap between the SHR we trained and the SHR of Lonca Company. How can I say this?
Well, if we use the recently popular bpfh rating as an example, the best rating we train is at the second-level level, while the worst one in Longka is also at the second-level level, and they are generally first-level."
When Bai Ye heard this, it was no wonder that Zhang Hanlin kept buying rats from abroad. The original reason was this. Thinking of this, he couldn't help but ask: "Then there are no other rats? To replace this kind of rat."
Dong Cunguang suddenly smiled, which was rare: "Yes! But... it's immature, and it's still under research."
Bai Ye suddenly realized that this was the first time he had laughed since he met Dong Cunguang, right?
"What can I do?"
Dong Cunguang's eyes were full of light: "In genetic engineering, I have recently been studying the genetic structure sequence of shr. If I can find the difference between him and other mice, we may be able to learn from it and use the shotgun method to change the characteristics of ordinary embryos at the embryonic level.
From the genetic sequence, we may be able to get something new.”
After Dong Cunguang finished speaking, he was full of expectations: "One day, we will break this disguised technology monopoly and let foreigners buy our mice!"