Yang Ping is very relieved that Lu Xiaolu is the director of the tumor laboratory. Lu Xiaolu's research field itself is the basic research of tumors, and this person is indeed a rare talent. The achievements he achieved before were achieved under a state of depression. Now he is completely physically and mentally.
In a liberated state, it is easier to achieve scientific research results.
After returning home from get off work, Yang Ping played with his son for a while. After changing his diaper, he held him in his arms and told him what operations he had done in the hospital. Hearing this, he was all smiles, and his limbs were kicking around with excitement.
Xiao Su is exercising in the gym at home. Her postpartum recovery is going very well. Yang Ping's mother and Mrs. Su are working together in the kitchen to cook. Professor Su and Yang Ping's father are chatting in the living room, as if they are commenting on a war movie. The family is getting along.
Very harmonious.
The advantage of having a system is that you can start work at any time. After Yang Ping told his son a story, he put him on the crib. The little guy blinked his eyes. It may have been hard. He blinked a few times and fell asleep. At this time, Xiao Su finished exercising.
.
"Let me watch him while you take a rest." Xiao Su said.
''I'll just take a nap here for a while," Yang Ping said.
Xiao Su knew Yang Ping's habits. He liked to take advantage of fragmented time to rest, so he was as energetic as a cow all day long.
Yang Ping just squinted on the chair in the bedroom for a while, and then did what he said. He used his spare time to enter the system space laboratory, and went to the system mall to purchase various equipment according to the needs of the experiment. Soon, all the hardware required for the experiment was completed.
To use M13 phage to carry K factor, we must first study the structure and function of M13 phage. Only by thoroughly studying it can we better transform it and make it work for ourselves.
Through the special camera system of the electron microscope, the M13 phage appeared on the electronic screen, showing a unique and fascinating shape.
From a morphological point of view, the M13 phage appears slender and uniform, like a delicate line. The ratio of its length to diameter makes it appear extremely eye-catching under the microscope. The shell is composed of the main coat protein p8, and these proteins are tightly arranged
Together, they form the protective layer of the phage. Under a microscope, you can clearly see the protein structures of these layers. They are arranged in an orderly manner and protect the internal genetic material of the phage.
For the first time, Yang Ping felt that the microbial structure assembled by this protein was so beautiful. Yang Ping couldn't help but marvel at the precision and beauty of living things. It even looked more harmonious and beautiful than any man-made machine.
The activity of M13 phage is also very distinctive. When they encounter a suitable host cell, such as E. coli containing F plasmid, they will demonstrate their ability to infect. Under a microscope, M13 phage can be observed passing through its secondary shell
Protein p3 attaches to the F pilus of E. coli, which is the first step in infection. Once attached, the M13 phage begins injecting its DNA into the host cell.
After injecting DNA, M13 phage will use the resources of the host cell to replicate. Under a microscope, you can observe that the interior of the infected cell begins to change, and new phage particles are gradually assembled in the cell. This process is very important to the life of the phage.
Cycles are crucial and key to their reproduction in nature.
It is worth noting that after infecting host cells, M13 phages do not cause host cell lysis like some phages. Instead, they secrete new phage particles from the infected cells, and the host cells are still able to continue to grow and
Split. This characteristic makes M13 phage uniquely valuable in biological research. But for Yang Ping, this is not an advantage, but a disadvantage.
The electronic screen continues to live broadcast to observe the morphology and activities of M13 phage under the microscope, which is like a fascinating journey of exploration. Their exquisite structure, unique infection method and distinctive life cycle all demonstrate the wonders and biology of nature.
The charm of diversity.
Such a simple virus is so sophisticated, so you can imagine how complex the human body is.
Looking at the phage on the screen, Yang Ping fell into thinking. He couldn't wait to complete the entire experiment quickly, but he didn't expect that a whole new world would open up when he started over.
If it is transformed into a killer that can infect tumor cells, they will no longer infect E. coli, but will accurately adsorb to the outer membrane of tumor cells, and then inject not only DNA through their long filamentous tails, but
DNA and K factor, K factor begins to work inside the cell, initiating the apoptosis of tumor cells, while DNA allows the phage to divide and grow in strength.
If M13 phages can be modified according to their goals, they will exhibit unique characteristics. They are no longer simple parasites, but become "intelligent warriors" that can actively attack tumors. This process involves sophisticated genetic engineering.
Allowing these tiny organisms to play a vital role in the battle against cancer.
However, the transformation process is destined to be difficult and complicated. The process of transforming M13 phage is complex and delicate.
First, it is necessary to determine which genes or proteins need to be modified or added to achieve anti-tumor effects, which usually involves precise editing of the phage genome, including inserting, deleting or replacing specific gene segments.
But for Yang Ping, it obviously does not need to be so complicated, because he has already mastered the K factor that initiates tumor cell apoptosis. He only needs to combine the K factor with the adhesion protein on the surface of the M13 phage, and then allow the phage to recognize and contact the tumor cells.
The rest of the work is done. If it is done more advanced, the K factor and the DNA inside the phage are mixed together. After the phage infects the tumor cells, the K factor mixed with the DNA is injected into the tumor cells together.
This chapter is not finished yet, please click on the next page to continue reading the exciting content! K factor can initiate the apoptosis of tumor cells and eventually kill the tumor cells. In this process, DNA can also waste materials inside the cells, which is harmful to
The phage itself divides and grows stronger.
After designing the experiment, Yang Ping handed over the rest of the work to the laboratory's robots, asking them to continuously modify the phage, and then conduct a pairing test with the phage and K factor. It is estimated that the M13 phage can obtain hundreds of millions of different phages through modification.
Which of the phages can adhere to K factor and which one can recognize tumor cells is a huge trial and error project. Trial and error is the specialty of the system space laboratory. Yang Ping left it to automated equipment to complete.
After coming out of the system space, Yang Ping yawned. Dinner was probably not ready yet. Xiao Su asked curiously: "Why do you feel like you have been injected with chicken blood after just sleeping for a while?"
"I'm thinking about a problem." Yang Ping said.
"What's the problem?" Xiao Su asked curiously.
"I was thinking about how phages inject DNA into cells. The long tail of the phage is like a thick tube. When it finds the cell it likes, it immediately locks on it, and then inserts the tail into the cell to forcefully inject the DNA.
Inject into cells, I have been thinking about this problem just now, so the more I think about it, the more excited I am." Yang Ping has indeed been thinking about this problem just now.
Xiao Su listened and wondered why this description was getting more and more wrong. Then if she realized something, she hit Yang Ping's chest with her pink fist.
"You are so energetic! After a whole day of surgery, you are still thinking about injecting DNA?"
"Honey, I was really just thinking about injecting DNA into your body. Oh, no, I meant how bacteriophages inject DNA into cells." Yang Ping got excited and confused his words.
Misunderstanding, wife!
My thoughts are pure, and I was really thinking about scientific research just now.
