The Virus Laboratory of Nandu Medical University is a level three biosafety laboratory, also known as a P3 laboratory.
According to the infectivity and harmfulness of pathogenic microorganisms, biosafety laboratories are internationally divided into four biosafety levels: P1, P2, P3 and P4. The so-called P4 laboratory is the laboratory with the highest biosafety level currently owned by mankind.
, known as the "aircraft carrier" in the field of virology research.
The P4 laboratory of Nandu Medical University is under construction. Once completed, it will be the second P4 laboratory in China.
A week later, visible turbidity appeared in the sterile transparent culture solution in the laboratory. When seeing the turbidity, the researcher wearing protective clothing was extremely excited.
These turbidities are nothing but the cultured K-virus complex. Yang Ping temporarily calls his combination of virus and K-factor the K-virus complex.
The researchers used special tools to carefully remove a portion of the virus-containing liquid. Although these viruses were not contagious and had been castrated and non-replicative, they still strictly followed the standard experimental procedures and did not dare to make any mistakes.
negligence.
These samples will be subjected to the most stringent testing to see whether they are pure, whether they have mutated, or whether they are mixed with other harmful microorganisms.
Viruses are microorganisms with the ability to replicate themselves, so we must be cautious when dealing with them. Of course, as long as these viruses do not undergo rare mutations, they are still safe and do not pose any danger to the human body. At most, they may cause some transient mild flu symptoms.
Moreover, this symptom is self-healing and will not leave any sequelae after self-healing. This is why some viruses can be used to treat tumors.
Some samples were specially processed and sent to an electron microscope for observation to analyze their structure.
The basic unit of structure and function of life is the cell, but viruses are an exception. They are non-cellular life and do not have a cellular structure. They are just a simple protein structure. Of course, the simplicity here is only relative to the complexity of cellular organisms.
Because the virus has no cellular structure, it must be parasitic in living cells to survive and reproduce. It cannot live independently and must rely on host cells to provide necessary life activity conditions. It operates through processes such as adsorption, injection, replication, combination and release.
Reproduce within host cells.
Human beings have been dealing with countless viruses, bacteria and other microorganisms since their birth. After a long period of evolution and evolution, some microorganisms have become part of our bodies.
For example, the organelles in human cells---mitochondria.
Mitochondria are organelles in almost all eukaryotic cells. They began as free-living viruses in ancient times, capable of producing energy in the presence of oxygen; they were then engulfed by ancestral eukaryotic cells, when these viruses parasitized ancestral eukaryotic cells.
After being in the hospital for too long, I gradually forgot my original identity and became an indispensable energy generator in the cell - mitochondria, which are the place of cellular respiration and many important metabolic processes.
Professor Zhang Zhiwei was an outstanding virologist that President Rao invited back from the United States. At that time, Professor Zhang returned to China as part of the Thousand Talents Program and came to Nandu Medical University to preside over virology research, which pushed China's virology research to the top in the world.
He is a pure virologist and doesn't know much about using viruses to treat tumors. But when he heard that Yang Ping was going to use the cultured new virus directly for human experiments, he couldn't help but feel a little worried because it violated the ethical principles of scientific research.
, once something happens, the consequences will be unimaginable, and everyone will be involved.
When Professor Zhang heard that Yang Ping from Sanbo Hospital wanted to cooperate with him, he initially wanted it. Later, after learning about the situation, he couldn't help but feel guilty. This kind of thing can be avoided and it is best to avoid it. Some ethical rules should not be challenged at will, otherwise it will be very difficult.
It is easy to put yourself in a situation of no return, there is no need to take such risks.
However, after a face-to-face conversation with Yang Ping, Professor Zhang changed his mind and developed admiration and respect for Yang Ping. Yang Ping shamed him with his selfless courage for science, so he finally agreed to cooperate.
However, for the sake of safety, Professor Zhang has extremely strict requirements for the researchers in the team when it comes to the details of the experiment. All researchers without three years of virus research experience are prohibited from participating in this experiment.
Although these viruses are relatively safe, they were transformed by Yang Ping after all. Yang Ping combined the virus with K factor to form a brand new virus. Although this virus still maintains the structure and structure of the original virus in terms of its main structure.
Function, the function of embedded K factor is very single, and it only targets one type of tumor cells.
Professor He Zhiwei is not worried about what evil the virus will do. He is worried about the K factor. This protein structure did not exist in the world before. It was cultivated out of thin air by Yang Ping in the laboratory. It is not a virus, but a protein molecule. There is no
Even though they have the ability to reproduce themselves, they cannot cause much trouble.
But the K factor is different after it combines with the virus. Although it is not a virus, it has now become part of the virus, that is, it can now divide along with the virus, which is very scary.
K factor can initiate the apoptosis program of tumor cells and cause the death of tumor cells. If it gets out of control, it can initiate the apoptosis program of normal cells. After being injected into the human body, it will attack human cells and various human cells indiscriminately.
Including cells in important organs, such as the brain, heart, lungs, liver, and kidneys, large-scale cell death will quickly cause organ failure, and the patient will die.
For this reason, Yang Ping also considered this issue when designing the experimental plan. In order to avoid the experiment getting out of control, he castrated these viruses, that is, castrated their replication ability, so that no matter how destructive they were, they would not be enough.
Fear, because it cannot be divided, so there is only as much as there is, and the combat effectiveness is extremely limited, and no matter how destructive these viruses are, they will inevitably be wiped out.
This chapter is not over yet, please click on the next page to continue reading! In the laboratory, a small amount can be injected into the human body first. If unexpected damage occurs, stop the injection and get symptomatic treatment immediately. The virus will slowly die in the human body, which is much safer.
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This technology is very mature and can be used to emasculate the replication ability of viruses. For example, this technology is used when manufacturing vaccines, such as viral vector vaccines.
It is a vaccine prepared using viruses (such as adenovirus) as vectors. These viruses as vectors will have the genes required for replication knocked out in advance, so that they can infect human cells but cannot replicate. The target pathogens are
The gene fragment corresponding to the specific protein is inserted into the genome of the vector virus, and the prepared vaccine is injected into the human body to infect the body cells and express the specific protein in the cells. This induces both humoral immunity and cellular immunity.
Allow the body to obtain higher immunogenicity.
This process is actually similar to the principle of Yang Ping's current experiment, so sometimes cross-disciplinary integration can create something new. Some things that were originally applied in another field may be able to be used in new fields.
Huge new features.
The first batch of experimental viruses have used genetic recombination technology to knock out the genes required for replication, and they have been "sterilized". Therefore, even if they have extremely rare mutations, they cannot inherit the mutations, and the mutated individuals are also very difficult to pass on.
Will die soon.
Professor Zhang Zhiwei personally participated in the experiment. He conducted electron microscopy observations on the sampled virus samples and found no virus mutations for the time being, indicating that the purity met the requirements of the experiment.
Speaking of viral mutations, not all mutations are bad for us, very rare mutations can be fatal but can also be beneficial, but most mutations have neither adverse nor beneficial effects, and some mutations do not even cause
Any change, this is called a "silent mutation".
There are many reasons for virus mutation, including replication errors, interaction with the host, and external conditions, such as ultraviolet rays. At present, the main cause of mutations in the laboratory is replication errors. After injection into the human body, based on the interaction with the human body,
Mutations will also occur, but these viruses are all castrated versions, so even mutations will have little effect.
Professor Zhang Zhiwei successfully obtained a certain number of samples, which temporarily met the experimental standards after testing. He couldn't wait to tell Yang Ping the good news.
After receiving the news, Yang Ping rushed to the laboratory of Nandu Medical University as quickly as possible. He wanted to see these K-virus complexes with his own eyes and see what they looked like. For them, Yang Ping made great efforts.
If everything goes well, he will next make the viruses into injections, and then use cold chain transportation similar to vaccines to transport them to the surgical institute. In the ward, Yang Ping will inject these brand-new viruses into Sisi through injection.
In vivo, close observation must be carried out after injection.
"I have been working on viruses for so many years, and I know that viruses are often used as delivery vehicles for vaccines. I never thought that this thing can also treat tumors?" Professor Zhang Zhiwei couldn't help but feel a little regretful.
Yang Ping told him: "I discovered the K factor first, and then chose to use the virus as a delivery vehicle. If there is no K factor, the virus as a delivery vehicle is meaningless."
Professor Zhang Zhiwei thought about it and realized that what Yang Ping said was right. In fact, the focus of his subject was not on viruses as delivery vehicles. This is an old method that has been used in many fields. It focused on the K factor, which has the most technical content.
The key is that it can initiate the apoptosis program of tumor cells and kill tumor cells. This is the biggest discovery, and it can be said to be a revolutionary discovery in the field of oncology.
"These viruses will be made into injectable biological agents as soon as possible and then sent to our department. I want to carry out human experiments as soon as possible." Yang Ping confirmed that these viruses met the experimental requirements.
"Does it have to be so urgent?" Professor Zhang Zhiwei kept thinking, always feeling that things were too rushed. He felt that there was a problem in a certain link, and he felt uneasy, but he couldn't think of any link that had the problem.
"Yes, my patients don't have time to wait too long, I have to race against time." Yang Ping told him truthfully.
Professor Zhang Zhiwei said doubtfully: "I really want to know what kind of patient is worthy of your risk. In fact, I personally think that you can just go step by step and not be impatient. Even if you miss this patient, there are many similar cases, and this one is not missing."
.”
Yang Ping didn't explain too much, and Professor Zhang Zhiwei didn't ask any more questions.
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Director Zhu completed his revenge. He did not leave China for Singapore immediately. He stayed to attend Yang Ping's wedding.
The overseas headquarters of Anning Group is located in Singapore. Since he was assigned to Singapore, he has been living in Singapore with his family and his children are also studying in Singapore. His family has settled in Singapore.
His battle with Japanese insurance companies finally came to an end. He defeated the Japanese and firmly occupied the Southeast Asian market. Now he is preparing to enter Europe and the United States and truly go global.
Therefore, from the perspective of the company's development, this competition with the Japanese was a life-or-death contest. He lived up to expectations and successfully defeated the Japanese and won this fierce business war.
For his personal hatred, he also successfully completed a wonderful revenge. After all these years, he finally found justice for himself. Thinking of his experiences over the years, he was full of emotions and felt a little uncomfortable in his heart, but he is very happy with his current life.
satisfy.
The Provincial People's Hospital's handling of his affairs was very wrong. They failed to withstand the pressure of public opinion and expelled Director Zhu abruptly even though they knew full well that he had been wrongly accused and framed.
Those hospital leaders did this out of complete ambiguity in order to keep their reputation. They were doctors. How could they not know that it was a colostomy surgery? How could they not know that the reporter was deliberately trying to slander Director Zhu? But they pretended to do so.
In order to put out the fire as soon as possible, they rudely fired Director Zhu to cope with public opinion, which made Director Zhu feel very chilled.
So since he was fired, Director Zhu has never set foot in the Provincial People's Hospital.
But now he wants to go back once. On behalf of Anning Insurance Company, he went to the Provincial People's Hospital to donate 200 million yuan to build advantageous disciplines. Anning Group is very generous and has donated money to several first-line hospitals in Nandu Province.
Director Zhu’s current annual salary is not only tens of millions of yuan, but he also has shares in the company. With annual salary and dividends, his annual income is already over 100 million yuan. Therefore, he can now be said to be one of the important figures in China’s insurance industry, truly pushing China’s insurance to the forefront.
The first person overseas.
Now that Director Zhu has returned to the Provincial People's Hospital, he can be regarded as returning home rich and powerful. He originally wanted to meet the dean who fired him back then, but unfortunately he did not meet him. The dean has been there for two years because of financial problems. Unexpectedly, a few years later,
There had been such a big change, and he suddenly felt like a world had changed.
He returned to his original department. The old director had retired and the current director was his brother who was about the same age as him. He looked at the ward that had not changed much. The doctor's office and duty room were still in their previous positions. Even the beds had not been changed. He