Chapter 79 Centrifugation

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 "Little Doctor Li?"

The old housekeeper of Duke Yan's mansion looked at Li Ang who suddenly visited with a look of shock.

"How is Duke Yan?"

Li Ang said: "I found a way."

"Please follow me."

The old housekeeper suddenly became serious and led Li Ang to the lobby.

You can still see medicine pots along the way and smell the lingering smell of medicine.

The descendants of Yan Mansion, including Yan Lin, were standing in the lobby, looking helplessly at Yan Yundang, who was sitting with his majestic horse and golden sword——

His face was still yellow, with his eyes closed, listening to what the man in Taoist robes in front of him was saying.

"...The general was born in Shuozhou, so he should naturally return to his roots. In the northwest of Shuozhou, there are two mountains, one is Pingding Xingshan, and the other is Gaoshan. There is a ditch in the middle called Dadi. The two mountains and one ditch store the wind and absorb the energy, so-called

If Qi rides on the wind, it will disperse; if it is bounded by water, it will stop..."

Okay, now you are looking at the feng shui of the cemetery.

Li Ang complained in his heart, but it was not very strange.

The average lifespan of the Yu people is not very high, and it is very rare to be seventy years old. Most elderly people will ask their families to prepare coffins, shrouds and graveyards early to prevent them from being buried in a hurry when they leave.
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The reason why Yan Lin and others are sad is entirely because Yan Yundang, as a martial arts master, fell ill too suddenly and did not give them any psychological preparation.

The housekeeper walked forward with gentle steps and said softly: "Lang Jun, little doctor Li is here."

"Huh?"

Yan Yundang opened his eyes, looked back at Li Ang, and said with a smile: "Master Li Xiaolang. The method you said last time is indeed good. Those pigeons will get sick if they only eat polished rice, and they will recover after eating brown rice.

It seems that there is really something wrong with my gastrointestinal tract and I cannot absorb that strange substance you mentioned."

"The Duke of Yan,"

Li Ang cupped his hands and said: "This junior is here for this - I have found a new way, which may be able to relieve the pain."

"What?!"

Before Yan Yundang said anything, Yan Lin took two steps forward with excitement on his face, "Doctor Li, do you really have a solution?"

Li Ang nodded and said: "It's just a method. The probability of successful cure is about 70%."

Yan Yundang's jaundice symptoms have not subsided, which proves that it is not pernicious anemia caused by a lack of folic acid. It is caused by vitamin b12.

"Seventy percent..."

Yan Lin turned to look at Yan Yundang. During this period, the Yan Mansion invited many famous doctors and prescribed some medicines including gastrointestinal treatment, but Yan Yundang's pernicious anemia still failed to improve.

"Father, you might as well give it a try."

"Yeah,"

Yan Yundang took a deep breath. Although he was ready to face death, if he could live one more day and watch his children and grandchildren grow up, he would be able to accept it. "Come here, wait for Dr. Li."

Prescription..."

"My lord, this medicine does not require a prescription."

Li Ang said: "The things I need are fresh pork liver, the more the better;

Boil cold water after cooling, the more the better;

Several bottles of pure wine produced by the Academy;

Four ceramic mortars; four thin balsa boards cut into circles; sixteen strong strings;

One drilling awl; eight chopsticks;

Four fine cloth bags;

A casserole and a porcelain bowl;

A cart of white soil;

In addition, we need thirty-six glass tubes - basically like this."

Li Ang asked the housekeeper for a pen and paper, and drew on the paper the top view, side view, and front view of the small glass test tube, and marked the dimensions on the three pictures.

Li Ang asked: "Can this kind of glass pipe be made with the level of craftsmen in Chang'an?"

"Yes."

Yan Lin took a look at it, stood up and said to Li Ang: "The best glass shops in Chang'an, Liulv Square, all enshrine telepathizers, who can use telepathy to change the shape of hot glass and create exquisite utensils."

"That's good."

Li Ang breathed a sigh of relief, "After all these are ready, we can start making medicine. By the way, General Yan, we still need four strong men and a light-tight room."

"Okay."

Yan Lin immediately asked his men to prepare - although these things are unrelated, but after seeing hundreds of pigeons fall ill and recover according to Li Ang's experimental method,

He only has trust in Li Ang.

"Little Doctor Li,"

Everyone in the Yan family became nervous and busy, but Yan Yundang still sat calmly on his seat and asked with some curiosity: "Isn't the montmorillonite white soil used to stop diarrhea? Does it need a whole cart?"<

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Li Ang shook his head, "This time it's not about stopping diarrhea, but filtering it."

Regarding Yan Yundang's illness, the entire Duke of Yan's government was operating at full capacity, and everything needed was quickly prepared for Li Ang.

"I need to prepare the medicine in a light-tight room. Please use straw mats or something to cover the windows outside.

If you want to watch from the sidelines, you can go into the house together, but you can only light an oil lamp."

Li Ang said casually and asked the servants of Yan Mansion to take all the utensils and enter the sealed room.

Yan Yundang followed him in curiously, but Yan Lin couldn't resist him, so he had no choice but to follow him with an oil lamp.

"The dosage of this medicine is quite large. I will only demonstrate it once. You should pay attention and remember it. It will be up to you to prepare the medicine for Duke Yan in the future."

Li Ang said to the Yan Mansion servants in the room, and by the light of the oil lamp in Yan Lin's hand,

First wash your palms with pure wine, then wash the fresh pork liver with pure wine to remove the blood stains on it.

Then pick up the scissors, cut the pig liver into pieces, put it into a mortar, grind it quickly and carefully, add water from time to time, and grind it further into chips.

The significance of this step is to crush the pig liver, which is rich in vitamin B12, and the next step is to centrifuge.

‘The normal laboratory centrifugation procedure is to pour the solution of pig liver fragments into a centrifuge tube and put it into a centrifuge. Spin it for ten minutes at a speed of 3500 rpm.

Separate the solid debris and liquid in the pig liver solution and obtain the supernatant with higher vitamin b12 content.

In the absence of a modern centrifuge, one can only make one...'

Li Ang took an awl and drilled four diagonally distributed button-shaped holes in the middle of the thin round wooden board. He passed two strings through the holes and looped them back, and fixed them on the chopsticks on both sides.
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Yan Lin wondered: "This is..."

"A rotating flywheel children's toy with centrifugal function."

Li Ang smiled and picked up two chopsticks, shook the string gently, pulled and retracted the chopsticks, and the round wooden board in the middle immediately started to rotate on its own.

Very good, the rotation is smooth.

Li Ang put down the flywheel toy with satisfaction, poured the pig liver solution into glass tubes of the same size, and plugged the corks, filling eight glass tubes in total.

And each glass tube is firmly tied to both sides of the circular flying disk by punching holes in the wooden board with ropes.

Achieve the same effect as the balance weight of a laboratory centrifuge.

Next...

Li Ang picked up the flywheel toy, held the chopsticks and pulled them back and forth, causing the circular wooden plate to continue spinning faster and faster.

This is the most primitive and simple artificial centrifuge in Li Ang's memory from another world, but the effect is not bad.

The centrifuge was invented by Saad Bhamla, a bioengineer at Georgia Tech.

Conventional centrifuges generate centrifugal force by rotating a drum, and separate the fluid into various layers in the sample tube through density. All centrifuges require electricity to operate, and are bulky and difficult to transport.

This artificial centrifuge, which imitates the principle of children's toys, can reach a maximum speed of thousands or even 10,000 using only ropes and plastics. It is the fastest rotating object driven by human power. It does not require the use of electricity at all.<

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The original intention of the invention of the artificial centrifuge was to solve the problem of blood testing in Africa, strive to separate plasma in the shortest time, and identify whether there is malaria parasite in the blood.

But now, it can also be used to centrifuge vitamin B12 in pig liver solution.

Li Ang unhurriedly pulled the wheel and asked the other four servants to imitate his own example, crushing the pig liver and centrifuging it.

Ten minutes later, Li Ang stopped pulling the wheel and took out the eight glass test tubes on both sides of the wooden disc.

Pour all the supernatant liquid into a casserole, pour a small amount of clay into it, and stir constantly.

White clay has a large specific surface area and pore volume, has special adsorption capacity and ion exchange properties, and can adsorb vitamin B12.

When the white clay has almost absorbed the solution, Li Ang puts the white clay into a clean fine cloth bag and drains the water.

Then pour the white clay adsorbed with vitamin B12 into a porcelain bowl, add water, and stir vigorously for 20 minutes.

Finally, filter the stirred solution and pour it out.

A high-content vitamin B12 solution was obtained—because vitamin B12 is easily decomposed when exposed to light, everything had to be done in a dark room.

"It's done."

Li Ang looked at the less than one bottle of clear liquid extracted from dozens of pieces of pig liver, breathed a long sigh of relief, and said to Yan Yundang: "This is the special substance extracted from pig liver.

This substance can relieve anemia and promote development - it can be given to poultry and livestock first, mixed with bran and fed for a long time. It should promote the rapid growth of livestock and observe the effect."

"No need."

Yan Yundang was also very straightforward. After looking at his set of procedures, he could see clearly that Li Ang did not add anything special to the solution.

Only the pig liver, water, and clay prepared by Yan Mansion will not be poisoned - and there is no need for it. He himself knows the severity of Yan Yundang's pernicious anemia.

"Give it to me."

Yan Yundang took the bottle and asked, "Can it be taken orally?"

"Just take it orally."

Li Ang nodded and said: "This medicine needs to be taken for life, once a day for seven days. After seven days, it should be taken every half month, and no process can be changed.

If it takes effect successfully, you will feel significantly better within two or three days, your appetite will increase, and your tongue pain will subside."

Oral vitamin B12 can also relieve pernicious anemia to a great extent, but the effect is not as good as intramuscular injection, so it must be taken in increasing amounts to prevent the recurrence of anemia.

"Should I take it for life..."

Yan Yundang grinned, "I don't know how many years I can live."

After saying that, he raised the bottle and drank it in one gulp, wiping the corners of his mouth.

"Father,"

Yan Lin subconsciously asked: "What does it smell like?"

"What else could it taste like? It tastes like blood and water."

Yan Yundang glanced at his son and muttered: "It's better than expected.

That’s right,”

He turned around and looked at Li Ang, "Little doctor Li, I heard that you were sentenced by Xi Yangyu to be unable to practice for life?"


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